Chen Jing, Li Chunlin, Yang Ji, Luo Zhenhua, Tang Songhua, Li Feng, Li Chunwang, Liu Bingwan, Jiang Zhigang
Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, No. 1 Beichen West Road, Beijing 100101, China.
Graduate School of the Chinese Academy of Sciences, No. 19 (A) Yuquan Road, Beijing 100049, China.
Int J Mol Sci. 2012;13(7):8805-8818. doi: 10.3390/ijms13078805. Epub 2012 Jul 16.
The three Procapra species, Tibetan gazelle (P. picticaudata), Mongolian gazelle (P. gutturosa) and Przewalski's gazelle (P. przewalskii) are endemic to Asia. Several intraspecific genetic issues have been studied with species-specific microsatellite loci in these Asian gazelles. However, cross-species microsatellite panels are absent, which inhibits comparative conservation and evolutionary studies of the Procapra. In this study, we isolated 20 cross-species microsatellite loci for Procapra from both related species and the genomic library of P. przewalskii. Fifty-three samples of the three gazelles were used to characterize the markers. Allele numbers ranged from three to 20, with a mean of 7.93 per locus. Observed heterozygosity (H(O)) averaged 0.680 and expected heterozygosity (H(E)) 0.767. The mean polymorphic information content (PIC) was 0.757 for P. picticaudata, 0.803 for P. gutturosa and 0.590 for P. przewalskii. Nine loci were significantly deviated from Hardy-Weinberg (H-W) equilibrium in the three species. Significant linkage disequilibrium was detected in four pairs of loci in P. przewalskii, five pairs in P. gutturosa and 51 pairs in P. picticaudata. Considering the abundance of published loci and their high success rates of cross-amplification, testing and utilization of loci from related species is efficient for wild species of Bovidae. The cross-species microsatellite loci we developed will facilitate further interspecies genetic studies in Procapra.
三种原羚物种，藏原羚（Procapra picticaudata）、蒙古原羚（Procapra gutturosa）和普氏原羚（Procapra przewalskii）均为亚洲特有物种。此前已利用这些亚洲原羚物种特异性微卫星位点对几个种内遗传问题进行了研究。然而，目前还没有跨物种微卫星面板，这阻碍了对原羚的比较保护和进化研究。在本研究中，我们从相关物种和普氏原羚的基因组文库中分离出20个用于原羚的跨物种微卫星位点。使用三种原羚的53个样本对这些标记进行了特征分析。等位基因数范围为3至20个，每个位点平均为7.93个。观察到的杂合度（H(O)）平均为0.680，预期杂合度（H(E)）为0.767。藏原羚的平均多态信息含量（PIC）为0.757，蒙古原羚为0.803，普氏原羚为0.590。在这三个物种中，有9个位点显著偏离哈迪-温伯格（H-W）平衡。在普氏原羚的4对位点、蒙古原羚的5对位点和藏原羚的51对位点中检测到显著的连锁不平衡。考虑到已发表位点的丰富性及其高交叉扩增成功率，利用相关物种的位点进行测试和利用对于牛科野生动物是有效的。我们开发的跨物种微卫星位点将有助于原羚进一步的种间遗传研究。
