The New Zealand Institute for Plant and Food Research Limited.
Cell Stress Chaperones. 2011 Nov;16(6):689-93. doi: 10.1007/s12192-011-0281-7. Epub 2011 Jul 13.
There is increasing evidence that mitochondrial dysfunction and oxidative stress may be integral to the pathogenesis of type 2 diabetes mellitus. Heat shock protein (Hsp60) is a mitochondrial stress protein known to be induced under conditions of mitochondrial impairment. Although this intracellular protein is normally found in the mitochondrion, several studies have shown that this protein is also present in systemic circulation. In this study, we report the presence of elevated levels of Hsp60 in both saliva and serum of type 2 diabetic patients compared to non-diabetic controls. Hsp60 was detectable in the saliva of 10% of control and 93% of type 2 diabetic patients. Levels detected were in the range of 3-7 ng/ml in control and 3-75 ng/ml in type 2 diabetic patients. Serum Hsp60 levels in the range of 3-88 ng/ml were detected in 33% of control subjects, and levels in the range of 28-1,043 ng/ml were detected in 100% of type 2 diabetic patients. This is the first reporting of the presence of mitochondrial stress protein in salivary secretions. The serum Hsp60 levels were 16-fold higher compared to those in saliva, and there was a good positive correlation between salivary and serum Hsp60 levels (r = 0.55). While the exact mechanisms responsible for the secretion of Hsp60 into biological fluids such as saliva and blood are not yet known. The presence of this molecular marker of mitochondrial stress in saliva offers a non-invasive route to further investigate the biological functions of extracellular Hsp60 in type 2 diabetes mellitus and other conditions.
越来越多的证据表明，线粒体功能障碍和氧化应激可能是 2 型糖尿病发病机制的重要组成部分。热休克蛋白（Hsp60）是一种已知在线粒体损伤情况下诱导产生的线粒体应激蛋白。虽然这种细胞内蛋白通常存在于线粒体中，但有几项研究表明，这种蛋白也存在于全身循环中。在这项研究中，我们报告了与非糖尿病对照组相比，2 型糖尿病患者的唾液和血清中 Hsp60 水平升高。在 10%的对照组和 93%的 2 型糖尿病患者的唾液中可检测到 Hsp60。对照组的检测水平为 3-7 ng/ml，2 型糖尿病患者为 3-75 ng/ml。在 33%的对照组中检测到血清 Hsp60 水平在 3-88 ng/ml 的范围内，在 100%的 2 型糖尿病患者中检测到 28-1043 ng/ml 的水平。这是首次报道线粒体应激蛋白存在于唾液分泌中。血清 Hsp60 水平比唾液中的水平高 16 倍，唾液和血清 Hsp60 水平之间存在良好的正相关（r=0.55）。虽然导致 Hsp60 分泌到唾液和血液等生物液中的确切机制尚不清楚。这种线粒体应激分子标志物在唾液中的存在为进一步研究 2 型糖尿病和其他情况下细胞外 Hsp60 的生物学功能提供了一种非侵入性途径。
