Traba Christian, Liang Jun F
a Department of Chemistry, Chemical Biology, and Biomedical Engineering, Charles V. Schaefer School of Engineering and Sciences , Stevens Institute of Technology , Hoboken , NJ , USA.
Biofouling. 2015;31(1):39-48. doi: 10.1080/08927014.2014.995643.
The direct application of low power argon plasma for the decontamination of pre-formed Staphylococcus aureus biofilms on various surfaces was examined. Distinct chemical/physical properties of reactive species found in argon plasmas generated at different wattages all demonstrated very potent but very different anti-biofilm mechanisms of action. An in-depth analysis of the results showed that: (1) the different reactive species produced in each plasma demonstrated specific antibacterial and/or anti-biofilm activity; and (2) the commonly associated etching effect could be manipulated and even controlled, depending on the experimental conditions. Under optimal experimental parameters, bacterial cells in S. aureus biofilms were killed (> 99.9%) by plasmas within 10 min of exposure and no bacteria nor biofilm regrowth from argon discharge gas treated biofilms was observed for 150 h. The decontamination ability of plasmas for the treatment of biofilm related contaminations on various materials was confirmed and an entirely novel layer-by-layer decontamination approach was designed and examined.
研究了低功率氩等离子体直接用于对各种表面上预先形成的金黄色葡萄球菌生物膜进行去污的效果。在不同功率下产生的氩等离子体中发现的活性物种具有独特的化学/物理性质，均显示出非常有效的但截然不同的抗生物膜作用机制。对结果的深入分析表明：（1）每种等离子体中产生的不同活性物种表现出特定的抗菌和/或抗生物膜活性；（2）根据实验条件，常见的蚀刻效应可以被操控甚至控制。在最佳实验参数下，金黄色葡萄球菌生物膜中的细菌细胞在暴露于等离子体10分钟内被杀死（>99.9%），并且在150小时内未观察到经氩放电气体处理的生物膜中有细菌或生物膜再生长。证实了等离子体对各种材料上与生物膜相关的污染物的去污能力，并设计和研究了一种全新的逐层去污方法。
