Al-Salam Suhail, Dhaheri Shamma Al, Awwad Aktham, Daoud Sayel, Shams Ahmed, Ashari Mouied Al
Pathology Department, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates.
Int J Pediatr Otorhinolaryngol. 2011 Sep;75(9):1160-6. doi: 10.1016/j.ijporl.2011.06.012. Epub 2011 Jul 12.
Given that Epstein-Barr virus (EBV) often inhabits human tonsils and adenoids, it remains to be distinctively determined its prevalence and in which cell and microenvironment the virus is present.
To determine the prevalence of EBV in the tonsils and adenoids of the United Arab Emirates (UAE) nationals and to provide a basis for understanding the origin and biology of EBV-infected cells, the immunophenotype of all EBV-infected cells in 46 tonsils and 46 adenoids was determined by EBER in situ hybridization and immunohistochemistry with monoclonal antibodies to T cells (CD3), B cells (CD20), and epithelial cells (cytokeratin AE1/AE3), as well as immunostaining with antibodies to EBV latent membrane protein-1 (LMP-1).
EBV was found in 43% of tonsillectomy specimens and 15% of adenoidectomy specimens. All EBV-infected cells were found to be B lymphocytes. About 90% of the infected B cells are found in the interfollicular regions of tonsils and adenoids and the remaining 10% are found within the follicles. There is no significant association between EBV infection, age (P=0.324) and gender (P=0.442).
EBV is associated with tonsillar hypertrophy and is prevalent in 43% of our cases. EBV is only detected in B lymphocytes and we believe that B lymphocytes are sites of primary infection and latency. In situ hybridization is the gold standard for the detection of EBV in tissue.
鉴于爱泼斯坦-巴尔病毒（EBV）常寄居于人类扁桃体和腺样体，其在这些组织中的流行情况以及病毒存在于何种细胞和微环境中仍有待明确确定。
为确定EBV在阿拉伯联合酋长国（UAE）国民扁桃体和腺样体中的流行情况，并为理解EBV感染细胞的起源和生物学特性提供依据，采用EBER原位杂交以及针对T细胞（CD3）、B细胞（CD20）和上皮细胞（细胞角蛋白AE1/AE3）的单克隆抗体进行免疫组化，同时使用抗EBV潜伏膜蛋白-1（LMP-1）抗体进行免疫染色，确定46个扁桃体和46个腺样体中所有EBV感染细胞的免疫表型。
在43%的扁桃体切除标本和15%的腺样体切除标本中发现了EBV。所有EBV感染细胞均为B淋巴细胞。约90%的感染B细胞位于扁桃体和腺样体的滤泡间区，其余10%位于滤泡内。EBV感染与年龄（P=0.324）和性别（P=0.442）之间无显著关联。
EBV与扁桃体肥大相关，在我们的病例中其流行率为43%。EBV仅在B淋巴细胞中被检测到，我们认为B淋巴细胞是原发性感染和潜伏的部位。原位杂交是检测组织中EBV的金标准。
