Centre de Recherche Biomoléculaire, Pietro Annigoni, Ouagadougou, Burkina Faso.
Trop Med Int Health. 2011 Nov;16(11):1392-6. doi: 10.1111/j.1365-3156.2011.02845.x. Epub 2011 Jul 18.
To determine hepatitis C virus (HCV) prevalence and the rate of HCV/human immunodeficiency virus (HIV) co-infection in pregnant women attending Saint Camille medical centre (SCMC) in Ouagadougou.
A total of 607 pregnant women, 16-45 years old, with <32 weeks amenorrhoea were screened for HCV and HIV using rapid tests. The majority of the women included in the study were previously known as HIV infected, as the centre is a reference centre for the programme of prevention against mother-to-child HIV transmission in the country. HCV RNA was extracted and quantified using the cDNA polymerase chain reaction with the nested primers at the 5' untranslated region. Transaminases were measured from plasma samples using spectrophotometric method.
Of women, 62.27% were infected with HIV. The prevalence of HCV was 2.14% in the screened pregnant women: 1.75% in HIV-negative women and 2.38% in HIV-positive ones. This prevalence is not significantly different between HIV-positive and HIV-negative pregnant women (P = 0.81). HCV RNA was found in all women with anti-HCV. A significant transaminase increase was noted in women infected with HCV (P = 0.01 and P < 0.01 for glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase, respectively). Risk factors significantly associated with HCV positivity in pregnant women included transfusion and genital excision. In addition, the infection was linked with the educational level of the women.
The issue of this study revealed that effort should be made to promote safe medical practices and fight against women genital excision that are found to be the main risk factors associated with the HCV infection.
确定在布基纳法索瓦加杜古圣卡米尔医疗中心（SCMC）就诊的孕妇中丙型肝炎病毒（HCV）的流行率和 HCV/人类免疫缺陷病毒（HIV）合并感染率。
共筛查了 607 名年龄在 16-45 岁、闭经时间<32 周的孕妇，采用快速检测法筛查 HCV 和 HIV。本研究中的大多数妇女以前都被认为是 HIV 感染者，因为该中心是该国预防母婴传播 HIV 方案的一个参照中心。采用巢式引物 5'非翻译区的 cDNA 聚合酶链反应提取并定量 HCV RNA。采用分光光度法测量血浆样本中转氨酶。
62.27%的妇女感染了 HIV。在筛查的孕妇中，HCV 的流行率为 2.14%：HIV 阴性妇女为 1.75%，HIV 阳性妇女为 2.38%。HIV 阳性和 HIV 阴性孕妇之间的这一流行率没有显著差异（P = 0.81）。在所有抗 HCV 阳性的妇女中均发现 HCV RNA。感染 HCV 的妇女转氨酶显著升高（谷氨酸-丙酮酸转氨酶和谷氨酸-草酰乙酸转氨酶分别为 P = 0.01 和 P < 0.01）。与孕妇 HCV 阳性相关的显著危险因素包括输血和生殖器切割。此外，该感染与妇女的教育程度有关。
本研究的问题表明，应努力促进安全医疗实践，并打击与 HCV 感染相关的主要危险因素，即妇女生殖器切割。
