亚油酸和γ-亚麻酸（月见草油）对大鼠肝脏脂肪酸结合蛋白的影响。

Effects of linoleic and gamma-linolenic acids (efamol evening primrose oil) on fatty acid-binding proteins of rat liver.

作者信息

Dutta-Roy A K, Demarco A C, Raha S K, Shay J, Garvey M, Horrobin D F

机构信息

Efamol Research Institute, Kentville, Nova Scotia, Canada.

出版信息

Mol Cell Biochem. 1990;98(1-2):177-82. doi: 10.1007/BF00231382.

DOI:10.1007/BF00231382

PMID:2176271

Abstract

We have studied the effects of Efamol evening primrose oil (EPO) on fatty acid-binding proteins (L-FABP) of rat liver. EPO contains 72% cis-linoleic acid and 9% cis-gamma linolenic acid. EPO has been clinically used for treatment of a number of diseases in humans and animals. EPO is also known to lower cholesterol level in humans and animals. Feeding of an EPO supplemented diet to rats (n = 9) for 2 months decreases the oleate binding capacity of purified L-FABP of rat liver whereas the palmitate binding activity was increased by 38%. However, EPO feeding did not alter the L-FABP concentrations significantly as measured by using the fluorescence fatty acid probe, dansylamino undecanoic acid. Endogenous fatty acid analysis of L-FABPs revealed significant qualitative and quantitative changes in fatty acid pattern after EPO feeding. EPO feeding decreased the endogenous palmitate level by 53% and oleate level by 64% in L-FABPs and also EPO feeding decreased the total endogenous fatty acid content from 62 nanomole per mg of protein to 42 nanomole per mg of L-FABP (n = 3).

摘要

我们研究了依芳莫 Evening Primrose 油（EPO）对大鼠肝脏脂肪酸结合蛋白（L-FABP）的影响。EPO 含有 72%的顺式亚油酸和 9%的顺式γ-亚麻酸。EPO 已在临床上用于治疗人类和动物的多种疾病。EPO 还已知可降低人类和动物的胆固醇水平。给大鼠（n = 9）喂食补充了 EPO 的饮食 2 个月，会降低大鼠肝脏纯化 L-FABP 的油酸结合能力，而棕榈酸结合活性则提高了 38%。然而，通过使用荧光脂肪酸探针丹磺酰氨基十一烷酸测量发现，喂食 EPO 并未显著改变 L-FABP 的浓度。对 L-FABP 的内源性脂肪酸分析显示，喂食 EPO 后脂肪酸模式在质量和数量上都有显著变化。喂食 EPO 使 L-FABP 中的内源性棕榈酸水平降低了 53%，油酸水平降低了 64%，并且喂食 EPO 还使内源性脂肪酸总含量从每毫克蛋白质 62 纳摩尔降至每毫克 L-FABP 42 纳摩尔（n = 3）。