Ramalingam L, Messer L B, Reynolds E C
School of Dental Science, and honorary dental officer, Royal Children's Hospital, Melbourne, Australia.
Pediatr Dent. 2005 Jan-Feb;27(1):61-7.
Enamel erosion can occur with frequent consumption of sports drinks. The purpose of this study was to determine a minimal concentration of casein phosphopeptide-stabilized amorphous calcium phosphate (CPP-ACP) which when added to a sports drink would eliminate such erosion in vitro.
Human enamel specimens were immersed in: (1) the sports drink Powerade; (2) Poweradeplus 4 concentrations of CPP-ACP (0.063%, 0.09%, 0.125%, 0.25%); or (3) double deionized water. Windows of test and control enamel were profiled, and the enamel surface characteristics were examined under scanning electron microscopy (SEM).
The pH of test solutions increased and the titratable acidity decreased with increasing CPP-ACP concentrations. Erosive step lesions occurred in specimens immersed in Powerade (mean depth=38.70kA +/- 5.60), which were eliminated by the addition of CPP-ACP to Powerade at all test concentrations except 0.063% CPP-ACP. Microscopic surface irregularities on test enamel were observed, apparent as adherent granules or globules. These may represent redeposited mineral phases following mobilization of calcium and phosphate from CPP-ACP. Tasters in a taste panel could not distinguish Powerade from Powerade plus 0.125% CPP-ACP.
Adding casein phosphopeptide-stabilized amorphous calcium phosphate to the sports drink Powerade significantly reduced the beverage's erosivity without affecting the product's taste.
频繁饮用运动饮料可能会导致牙釉质侵蚀。本研究的目的是确定酪蛋白磷酸肽稳定的无定形磷酸钙(CPP-ACP)的最低浓度,将其添加到运动饮料中可在体外消除此类侵蚀。
将人类牙釉质标本浸入:(1)运动饮料Powerade;(2)添加4种浓度CPP-ACP(0.063%、0.09%、0.125%、0.25%)的Powerade;或(3)双去离子水。对测试和对照牙釉质的窗口进行轮廓分析,并在扫描电子显微镜(SEM)下检查牙釉质表面特征。
随着CPP-ACP浓度的增加,测试溶液的pH值升高,可滴定酸度降低。浸入Powerade中的标本出现侵蚀性阶梯状病变(平均深度=38.70kA±5.60),除0.063% CPP-ACP外,在所有测试浓度下向Powerade中添加CPP-ACP均可消除此类病变。观察到测试牙釉质表面有微观不规则现象,表现为附着的颗粒或小球。这些可能代表从CPP-ACP中动员钙和磷酸盐后重新沉积的矿相。味觉小组的品尝者无法区分Powerade和添加0.125% CPP-ACP的Powerade。
向运动饮料Powerade中添加酪蛋白磷酸肽稳定的无定形磷酸钙可显著降低饮料的侵蚀性,且不影响产品口感。