纳米碳酸钙对预防牙齿漂白后再染色的体外研究。

Effect of nano-carbonate apatite to prevent re-stain after dental bleaching in vitro.

机构信息

Department of Preventive Dentistry and Public Oral Health, Research Center for Orofacial Hard Tissue Regeneration Brain Korea 21 project, College of Dentistry, Yonsei University, Seoul, Republic of Korea.

出版信息

J Dent. 2011 Sep;39(9):636-42. doi: 10.1016/j.jdent.2011.07.002. Epub 2011 Jul 7.

DOI:10.1016/j.jdent.2011.07.002

PMID:21763391

Abstract

OBJECTIVES

This study examined the effect of nano-carbonate apatite (n-CAP) to prevent re-staining and the change of enamel surface after dental bleaching in vitro.

METHODS

Twenty-four bovine specimens were bleached for 2 weeks with 10% carbamide peroxide (CP). After bleaching, the specimens were divided into the following four groups: distilled and deionized water (DDW, negative control), 10% n-CAP, NaF (positive control) and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP, positive control). Each group was subjected to pH cycling for 7 days. The specimens were treated for 4 min 3 times per day and re-staining was induced naturally by artificial saliva in the remineralization process. After pH cycling, the changes in colour were evaluated with spectrophotometry and scanning electron microscopy (SEM). The difference in colour between before and after pH cycling was evaluated using an ANOVA and Tukey test.

RESULTS

After pH cycling, the colour difference of n-CAP group was significantly lower than that of the DDW and CPP-ACP groups (p<0.05). SEM showed that n-CAP particles were deposited regularly on the damaged surface compared to the other groups.

CONCLUSION

10% n-CAP could significantly maintain the initial colour and protect the damaged enamel structure after bleaching.

摘要

目的

本研究旨在探讨纳米碳酸钙磷灰石（n-CAP）对预防牙齿漂白后再染色和牙釉质表面变化的效果。

方法

将 24 个牛标本用 10%过氧化脲（CP）漂白 2 周。漂白后，将标本分为以下四组：蒸馏水和去离子水（DDW，阴性对照）、10%n-CAP、氟化钠（阳性对照）和酪蛋白磷酸肽-无定形磷酸钙（CPP-ACP，阳性对照）。每组进行 7 天的 pH 循环。标本每天处理 4 分钟 3 次，在再矿化过程中通过人工唾液自然诱导再染色。pH 循环后，用分光光度法和扫描电子显微镜（SEM）评估颜色变化。用方差分析和 Tukey 检验评估 pH 循环前后颜色差异。