Department of Ophthalmology, University Hospital of Patras, Patras, Greece.
Am J Ophthalmol. 2011 Nov;152(5):824-33.e1. doi: 10.1016/j.ajo.2011.04.009. Epub 2011 Jul 20.
To construct a model simulating intraocular lens (IOL) opacification attributable to the formation of calcium phosphate deposits and to investigate the kinetics of deposit formation.
Prospective laboratory investigation.
Department of Ophthalmology, Medical School and Department of Chemical Engineering, Laboratory of Inorganic and Analytical Chemistry, University of Patras, Greece.
Three hydrophilic acrylic IOLs (26% water content) were placed inside a 10-mL double-walled thermostated reactor simulating the anterior chamber. Simulated aqueous humor was injected continuously into the reactor using a pump with variable speed.
The observation of IOLs was carried out in situ daily by optical microscopy. Scanning electron microscopy and energy-dispersive radiographic spectroscopy were used for the identification of the morphologic features and the composition of the deposits.
The lenses were removed and inspected 5, 9, and 12 months after the initiation of the experiment. Investigation showed deposits of calcium phosphate crystallites in the interior of opacified IOLs. However, these deposits were not observed on the surface of the IOLs.
In agreement with earlier reports by our group and in the literature, IOL opacification is the result of calcification. It is suggested that the surface hydroxyl groups of the polyacrylic polymeric components of the IOLs are capable of inducing surface nucleation and crystal growth of calcium phosphates. However, most important is the finding that the calcification of IOLs is initiated from their interior through the development of sufficiently high local supersaturation, realized through the diffusion of calcium and phosphate ions.
构建一个模拟因钙磷酸盐沉淀形成而导致的人工晶状体（IOL）混浊的模型，并研究沉淀形成的动力学。
前瞻性实验室研究。
希腊帕特雷大学医学院眼科系和化学工程系无机及分析化学实验室。
将 3 个亲水丙烯酸人工晶状体（26%含水量）置于模拟前房的 10 毫升双层恒温反应器内。使用变速泵持续将模拟房水注入反应器。
通过光学显微镜对人工晶状体进行原位每日观察。使用扫描电子显微镜和能量色散射线光谱法鉴定沉淀物的形态特征和成分。
实验开始后 5、9 和 12 个月，将晶状体取出并进行检查。研究表明，混浊人工晶状体内部有钙磷酸盐晶体沉淀物。然而，在人工晶状体表面并未观察到这些沉淀物。
与我们小组和文献中的早期报告一致，IOL 混浊是钙化的结果。研究表明，IOL 聚合成分的表面羟基基团能够诱导钙磷酸盐的表面成核和晶体生长。然而，最重要的发现是，IOL 的钙化是从内部开始的，通过钙和磷酸盐离子的扩散实现足够高的局部过饱和度。
