Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan.
Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, Taiwan.
Int J Syst Evol Microbiol. 2011 Aug;61(Pt 8):1887-1893. doi: 10.1099/ijs.0.024190-0. Epub 2010 Sep 10.
A Gram-negative, heterotrophic, marine bacterium, designated strain SW-11(T), was isolated from the reef-building coral Isopora palifera in Kenting, Taiwan. Cells were rods and were motile by a single polar flagellum. The strain grew at 10-45 °C (optimum, 30-35 °C), at pH 7.0-8.0 (optimum, pH 7.5) and with 2.0-4.0 % NaCl (optimum, 2.5-3.0 %). The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, diphosphatidylglycerol and four unknown phospholipids. Isoprenoid quinones consisted of ubiquinone 9 (78.8 %) and ubiquinone 8 (21.1 %). Major cellular fatty acids were summed feature 3 (C(16 : 1)ω7c and/or C(16 : 1)ω6c; 22.3 %), C(17 : 1)ω8c (13.4 %), summed feature 8 (C(18 : 1)ω6c and/or C(18 : 1)ω7c; 13.1 %), C(16 : 0) (10.3 %) and anteiso-C(17 : 1)ω9c (10.0 %). The DNA G+C content was 51.6 mol%. 16S rRNA gene sequence analysis indicated that strain SW-11(T) belongs to the class Gammaproteobacteria and is a member of the order Alteromonadales. Strain SW-11(T) shared 93.2 % 16S rRNA gene sequence similarity with Teredinibacter turnerae T7902(T) and 92.1 % with Saccharophagus degradans 2-40(T), and can be further distinguished from these two related strains by distinct patterns of fatty acid content and differences in the polar lipid profile, the ability to utilize different compounds as carbon sources, the ability to degrade various compounds and differences in enzyme activities. The phylogenetic data and those from physiological, morphological and chemotaxonomic characterizations indicate that strain SW-11(T) represents a novel species and genus, for which the name Pseudoteredinibacter isoporae gen. nov., sp. nov. is proposed. The type strain of Pseudoteredinibacter isoporae is SW-11(T) ( = BCRC 17935(T) = LMG 25246(T)).
一株革兰氏阴性、异养、海洋细菌，被命名为 SW-11(T) 菌株，从台湾垦丁的造礁珊瑚 Isopora palifera 中分离得到。细胞呈杆状，通过单一极生鞭毛运动。该菌株在 10-45°C（最佳温度为 30-35°C）、pH7.0-8.0（最佳 pH7.5）和 2.0-4.0%NaCl（最佳 2.5-3.0%）下生长。极性脂类由磷脂酰乙醇胺、磷脂酰甘油、磷脂酰丝氨酸、双磷脂酰甘油和四种未知磷脂组成。异戊二烯醌由泛醌 9（78.8%）和泛醌 8（21.1%）组成。主要细胞脂肪酸为 summed feature 3（C(16:1)ω7c 和/或 C(16:1)ω6c；22.3%）、C(17:1)ω8c（13.4%）、 summed feature 8（C(18:1)ω6c 和/或 C(18:1)ω7c；13.1%）、C(16:0)（10.3%）和 anteiso-C(17:1)ω9c（10.0%）。DNA G+C 含量为 51.6mol%。16S rRNA 基因序列分析表明，SW-11(T) 菌株属于γ变形菌纲，是交替单胞菌目（Alteromonadales）的成员。SW-11(T) 菌株与 Teredinibacter turnerae T7902(T) 的 16S rRNA 基因序列相似性为 93.2%，与 Saccharophagus degradans 2-40(T) 的相似性为 92.1%，并且可以通过脂肪酸含量的不同模式和极性脂谱的差异、不同化合物作为碳源的利用能力、不同化合物的降解能力和酶活性的差异与这两个相关菌株进一步区分。系统发育数据以及生理、形态和化学分类特征表明，SW-11(T) 菌株代表一个新的种和属，提议建立 Pseudoteredinibacter isoporae 属，新种。Pseudoteredinibacter isoporae 的模式菌株为 SW-11(T)（=BCRC 17935(T)=LMG 25246(T)）。
