Department of Physiology, School of Medicine, Tokai University, 143, Shimokasuya, Isehara, Kanagawa 259-1193, Japan.
J Clin Biochem Nutr. 2011 Jul;49(1):31-5. doi: 10.3164/jcbn.10-112.. Epub 2011 Jun 17.
Kurozu moromimatsu is the sediment of Kurozu, a jar-fermented Japanese black vinegar produced from unpolished rice. Here, we examined the protective effects of Kurozu moromimatsu in a diethylnitrosamine-induced model of hepatocellular carcinoma. Thirty-two F344 rats were divided into two groups; the control group received basal CE-2 diet, and the Kurozu moromimatsu group received CE-2 diet containing Kurozu moromimatsu. At 16 weeks after initial intraperitoneal administration of diethylnitrosamine (150 mg/kg/week), serum was collected from half the rats. These rats were sacrificed and the liver was resected for histological examination of hematoxylin-eosin-stained sections and assay of matrix metalloproteinase-2 and matrix metalloproteinase-9 levels in tumor tissues. Glutathione S-transferase placental form-positive foci were evaluated by immunostaining for glutathione S-transferase placental form. The remaining rats were maintained for evaluation of survival. There were no significant differences of serum transaminases, tumor necrosis factor-alpha, and also no marked hepatic histological differences, between the two groups. However, the size of hepatocellular carcinomas was greatly decreased and the levels of activated matrix metalloproteinase-2 and -9 were significantly reduced in the Kurozu moromimatsu group. Further, survival was significantly prolonged in the Kurozu moromimatsu group compared with the control. These results indicate that Kurozu moromimatsu inhibited the growth of hepatocellular carcinoma.
黑泽森是由未抛光的大米制成的日本黑醋经过发酵后形成的沉淀物。在这里，我们研究了黑泽森对二乙基亚硝胺诱导的肝癌模型的保护作用。将 32 只 F344 大鼠分为两组；对照组给予基础 CE-2 饮食，黑泽森组给予含有黑泽森的 CE-2 饮食。在初次腹腔注射二乙基亚硝胺（150mg/kg/周）16 周后，从一半大鼠中收集血清。这些大鼠被处死，切除肝脏，用于苏木精-伊红染色切片的组织学检查和肿瘤组织中基质金属蛋白酶-2 和基质金属蛋白酶-9 水平的测定。通过免疫组化法检测谷胱甘肽 S-转移酶胎盘型阳性灶来评估谷胱甘肽 S-转移酶胎盘型。其余大鼠继续饲养以评估其生存情况。两组间血清转氨酶、肿瘤坏死因子-α无显著差异，肝组织学差异也不明显。然而，黑泽森组肝癌的大小显著减小，活化的基质金属蛋白酶-2 和-9 的水平明显降低。与对照组相比，黑泽森组的生存率显著延长。这些结果表明黑泽森抑制了肝癌的生长。
