Department of Bioengineering, University of California, San Diego, La Jolla, 92093-0412, USA.
AAPS J. 2010 Dec;12(4):716-28. doi: 10.1208/s12248-010-9232-y. Epub 2010 Oct 19.
Nanotechnology is giving us a glimpse into a nascent field of nanopharmacology that deals with pharmacological phenomena at molecular scale. This review presents our perspective on the use of scanning probe microscopy techniques with special emphasis to multidimensional atomic force microscopy (m-AFM) to explore this new field with a particular emphasis to define targets, design therapeutics, and track outcomes of molecular-scale pharmacological interactions. The approach will be to first discuss operating principles of m-AFM and provide representative examples of studies to understand human health and disease at the molecular level and then to address different strategies in defining target macromolecules, screening potential drug candidates, developing and characterizing of drug delivery systems, and monitoring target-drug interactions. Finally, we will discuss some future directions including AFM tip-based parallel sensors integrated with other high-throughput technologies which could be a powerful platform for drug discovery.
纳米技术让我们得以一窥纳米药理学这一新兴领域，该领域涉及分子尺度上的药理学现象。本综述介绍了我们对扫描探针显微镜技术的应用的看法，特别强调多维原子力显微镜（m-AFM）在探索这一新领域的应用，特别强调确定靶点、设计治疗方法以及跟踪分子水平的药物相互作用的结果。方法是首先讨论 m-AFM 的工作原理，并提供代表性的研究实例，以了解分子水平上的人类健康和疾病，然后讨论定义靶标大分子、筛选潜在药物候选物、开发和表征药物传递系统以及监测靶标-药物相互作用的不同策略。最后，我们将讨论一些未来的方向，包括与其他高通量技术集成的基于 AFM 尖端的并行传感器，这可能是药物发现的强大平台。
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