De Souza Y G, Freese U K, Greenspan D, Greenspan J S
Oral AIDS Center, University of California, San Francisco 94143.
J Clin Microbiol. 1990 Dec;28(12):2775-8. doi: 10.1128/jcm.28.12.2775-2778.1990.
The presence of Epstein-Barr virus (EBV) DNA in the epithelial cells of oral hairy leukoplakia is the confirming criterion in the diagnosis of this lesion, which occurs mainly in persons infected by the human immunodeficiency virus. Because hairy leukoplakia often presages the development of the acquired immune deficiency syndrome, it is important that suspicious lesions be accurately diagnosed. Commonly, biopsy tissue is removed for detection of EBV DNA by in situ hybridization, but biopsy is contraindicated in some patients. This study evaluated filter and cytospin in situ hybridization, two noninvasive techniques that examine epithelial cells swabbed from the surfaces of the lesions, for their sensitivity in detecting EBV DNA. As compared with tissue in situ hybridization, the filter and cytospin techniques had sensitivities of 100 and 92%, respectively. We conclude that these two noninvasive techniques can provide the clinician with an accurate alternative to biopsy whenever this human immunodeficiency virus-associated lesion is suspected.
口腔毛状白斑上皮细胞中存在爱泼斯坦-巴尔病毒（EBV）DNA是诊断该病变的确切标准，该病变主要发生于感染人类免疫缺陷病毒的人群。由于毛状白斑常预示获得性免疫缺陷综合征的发展，准确诊断可疑病变很重要。通常，会切除活检组织通过原位杂交检测EBV DNA，但活检在某些患者中是禁忌的。本研究评估了滤膜和细胞离心涂片原位杂交这两种非侵入性技术，它们通过检测从病变表面擦拭的上皮细胞来检测EBV DNA，评估了它们在检测EBV DNA方面的敏感性。与组织原位杂交相比，滤膜和细胞离心涂片技术的敏感性分别为100%和92%。我们得出结论，每当怀疑存在这种与人类免疫缺陷病毒相关的病变时，这两种非侵入性技术可为临床医生提供一种准确的活检替代方法。
