Tate H, Kodama H, Izawa H
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Nihon Juigaku Zasshi. 1990 Oct;52(5):931-7. doi: 10.1292/jvms1939.52.931.
The infectivity of infectious pancreatic necrosis virus (IPNV) for rainbow trout (Oncorhynchus mykiss) mononuclear leukocyte subpopulations was investigated to determine the mechanisms of immunosuppression caused by the virus. IPNV was recovered from nylon wool-adherent, surface immunoglobulin (Ig)-positive leukocytes of head kidney, spleen and peripheral blood collected from virus-inoculated fish with higher titers than non-adherent, Ig-negative cells. Non-adherent cell population showed mitogenic response to phytohemagglutinin and concanavalin A but not to lipopolysaccharide. Conversely, the responses of adherent cells to these mitogens were weak. Mitogenic response and non-specific cytotoxicity of head kidney leukocytes significantly decreased by the inoculation of fish with the virus. These results suggest that the suppression of immune responses is involved in the establishment of carrier state in fish after infection with IPNV.
为了确定传染性胰腺坏死病毒（IPNV）引起免疫抑制的机制，研究了该病毒对虹鳟（Oncorhynchus mykiss）单核白细胞亚群的感染性。从接种病毒的鱼的头肾、脾脏和外周血中收集的尼龙毛黏附、表面免疫球蛋白（Ig）阳性白细胞中回收IPNV，其滴度高于非黏附、Ig阴性细胞。非黏附细胞群体对植物血凝素和刀豆球蛋白A有丝分裂原反应，但对脂多糖无反应。相反，黏附细胞对这些有丝分裂原的反应较弱。接种病毒后，头肾白细胞的有丝分裂原反应和非特异性细胞毒性显著降低。这些结果表明，免疫反应的抑制参与了IPNV感染后鱼类携带状态的建立。
