Ferreira Paulo Michel P, Farias Davi F, Viana Martônio P, Souza Terezinha M, Vasconcelos Ilka M, Soares Bruno M, Pessoa Cláudia, Costa-Lotufo Letícia V, Moraes Manoel O, Carvalho Ana F U
Departamento de Ciências Biológicas, Campus Senador Helvídio Nunes de Barros, Universidade Federal do Piauí, Picos, Brasil.
An Acad Bras Cienc. 2011 Sep;83(3):1045-58. doi: 10.1590/s0001-37652011005000017. Epub 2011 Jul 15.
This study assessed the antiproliferative and cytotoxic potential against tumor lines of ethanolic seed extracts of 21 plant species belonging to different families from Northeastern Brazil. In addition, some underlying mechanisms involved in this cytotoxicity were also investigated. Among the 21 extracts tested, the MTT assay after 72 h of incubation demonstrated that only the ethanolic extract obtained from Myracrodruon urundeuva seeds (EEMUS), which has steroids, alkaloids and phenols, showed in vitro cytotoxic activity against human cancer cells, being 2-fold more active on leukemia HL-60 line [IC(50) value of 12.5 (9.5-16.7) μg/mL] than on glioblastoma SF-295 [IC(50) of 25.1 (17.3-36.3) μg/mL] and Sarcoma 180 cells [IC(50) of 38.1 (33.5-43.4) μg/mL]. After 72h exposure, flow cytometric and morphological analyses of HL-60-treated cells showed that EEMUS caused decrease in cell number, volume and viability as well as internucleosomal DNA fragmentation in a dose-dependent way, suggesting that the EEMUS triggers apoptotic pathways of cell death.
本研究评估了来自巴西东北部不同科的21种植物种子乙醇提取物对肿瘤细胞系的抗增殖和细胞毒性潜力。此外，还研究了这种细胞毒性所涉及的一些潜在机制。在测试的21种提取物中，孵育72小时后的MTT分析表明，只有从含有类固醇、生物碱和酚类的乌伦杜瓦杨梅种子中获得的乙醇提取物（EEMUS）对人类癌细胞具有体外细胞毒性活性，对白血病HL-60细胞系的活性比对胶质母细胞瘤SF-295 [IC(50)值为25.1（17.3 - 36.3）μg/mL]和肉瘤180细胞[IC(50)值为38.1（33.5 - 43.4）μg/mL]高2倍[IC(50)值为12.5（9.5 - 16.7）μg/mL]。暴露72小时后，对HL-60处理细胞的流式细胞术和形态学分析表明，EEMUS以剂量依赖的方式导致细胞数量、体积和活力下降以及核小体间DNA片段化，表明EEMUS触发了细胞死亡的凋亡途径。
