Institute of Agro-food Science and Technology of Chinese Academy of Agricultural Sciences, 2nd Yuanmingyuan West Road, Hai Dian District, Beijing 100193, PR China.
J Hazard Mater. 2011 Sep 15;192(3):1192-202. doi: 10.1016/j.jhazmat.2011.06.027. Epub 2011 Jun 17.
The identification of the radiolytic products of mycotoxins is a key issue in the feasibility study of gamma ray radiation detoxification. Methanol-water solution (60:40, v/v) spiked with aflatoxin B(1) (AFB(1); 20 mg L(-1)) was irradiated with Co(60) gamma ray to generate radiolytic products. Liquid chromatography-quadruple time-of-flight mass spectrometry was applied to identify the radiolytic products of AFB(1). Accurate mass and proposed molecular formulas with a high-matching property of more than 20 radiolytic products were obtained. Seven key radiolytic products were proposed based on the molecular formulas and tandem mass spectrometry spectra. The analyses of toxicity and formation pathways were proposed based on the structure of the radiolytic products. The addition reaction caused by the free-radical species in the methanol-water solution resulted in the formation of most radiolytic products. Based on the structure-activity relationship analysis, the toxicity of radiolytic products was significantly reduced compared with that of AFB(1) because of the addition reaction that occurred on the double bond in the terminal furan ring. For this reason, gamma irradiation is deemed an effective tool for the detoxification of AFB(1).
真菌毒素辐射解毒可行性研究的关键问题是鉴定其辐射分解产物。用 Co(60)γ射线辐照甲醇-水（60:40，v/v）溶液，溶液中含有黄曲霉毒素 B(1)（AFB(1)；20mg/L），以生成辐射分解产物。采用液相色谱-四极杆飞行时间质谱联用技术鉴定 AFB(1)的辐射分解产物。获得了 20 多种具有高匹配性的精确质量和提出的分子公式的辐射分解产物。基于分子公式和串联质谱图谱，提出了 7 种关键的辐射分解产物。根据辐射分解产物的结构提出了毒性和形成途径的分析。甲醇-水溶液中的自由基物质的加成反应导致了大多数辐射分解产物的形成。基于结构-活性关系分析，由于末端呋喃环上的双键发生加成反应，辐射分解产物的毒性明显低于 AFB(1)。因此，γ辐照被认为是 AFB(1)解毒的有效工具。
