Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
J Proteomics. 2011 Nov 18;74(12):2837-45. doi: 10.1016/j.jprot.2011.06.026. Epub 2011 Jul 18.
The yeast Saccharomyces cerevisiae has been used as a model organism to investigate responses to different environmental stressors. The importance of their conclusions has been expanded to human cells. The experiments were done with exponentially growing cells, which do not resemble human cells. Human and other eukaryotic cells spend the greater part of their lives in a quiescent state, known as G0 corresponding to the yeast stationary phase. Providing energy, which comes from mitochondrial respiration, is also common. Thus, in the present study S. cerevisiae was used in the stationary phase for characterization at the cellular and proteome levels. At the cellular level, optical density, cell viability, glycogen content, intracellular oxidation and cell energy metabolic activity were measured, while at the proteome level, protein profiles were analyzed using two-dimensional electrophoresis. The data obtained at both levels provide better insight into quiescence program state, which still remains poorly understood. At their base, optimal time period reflecting a stable metabolic and oxidative state of the yeast was determined. Consequently, this period is the appropriate to study changes in cell oxidant status and energy metabolic activity in response to different environmental stressors.
酵母酿酒酵母已被用作研究不同环境胁迫反应的模式生物。其结论的重要性已扩展到人类细胞。这些实验是在指数生长期的细胞中进行的，这些细胞与人类细胞并不相似。人类和其他真核细胞在静止状态下度过大部分生命，称为 G0，对应于酵母的静止期。提供能量，这来自线粒体呼吸，也很常见。因此，在本研究中，使用处于静止期的酿酒酵母进行细胞和蛋白质组水平的表征。在细胞水平上，测量了光密度、细胞活力、糖原含量、细胞内氧化和细胞能量代谢活性，而在蛋白质组水平上，使用二维电泳分析了蛋白质图谱。在这两个水平上获得的数据提供了对静止程序状态的更好理解，而静止程序状态仍然知之甚少。在其基础上，确定了反映酵母稳定代谢和氧化状态的最佳时间段。因此，这个时间段是研究细胞氧化剂状态和能量代谢活性对不同环境胁迫反应的合适时间。
