Berg Lise C, Thomsen Preben Dybdahl, Andersen Pia Haubro, Jensen Henrik Elvang, Jacobsen Stine
Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Gronnegaardsvej 7, 1870 Frederiksberg C, Denmark.
Vet Immunol Immunopathol. 2011 Nov 15;144(1-2):155-9. doi: 10.1016/j.vetimm.2011.06.037. Epub 2011 Jul 5.
mRNA expression of the acute phase protein serum amyloid A (SAA) in histologically normal tissues derived from horses (n=13) and cattle (n=4) was investigated by quantitative reverse-transcriptase real-time polymerase-chain reaction. As expected, high constitutive SAA mRNA expression was demonstrated in hepatic tissue in both species. In horses, moderate (>1% of the hepatic expression) SAA mRNA expression was detected in the lung, mammary gland, pancreas, synovial membrane, thymus, thyroid gland and uterus. Other equine tissues and organs sampled included adipose tissue, adrenal gland, aorta, brain, different gastro-intestinal tissues, heart, kidney, lymph node, ovary, testis, prostate, skeletal and cardiac muscle, skin and spleen; all showed low (<1% of the hepatic expression) SAA mRNA expression. In cattle, SAA mRNA was expressed in moderate levels in adipose tissue, colon, jejunum, mammary gland, skeletal muscle, synovial membrane, thymus, thyroid gland, and uterus; expression was low in the remainder of the samples (same tissue panel as horses). The results confirm the liver as the main site of SAA production. Even though there was some inter-species variation in tissues expressing SAA mRNA, several organs communicating with the external environment (lung, mammary gland, uterus, and certain parts of the gastro-intestinal tract) showed SAA mRNA expression, which supports the hypothesis that SAA might possess a role in the innate defence against invading pathogens. The results of the study thus warrant further studies into functions of hepatically and extrahepatically produced SAA isoforms.
通过定量逆转录实时聚合酶链反应，研究了源自马（n = 13）和牛（n = 4）的组织学正常组织中急性期蛋白血清淀粉样蛋白A（SAA）的mRNA表达。正如预期的那样，在两个物种的肝组织中均显示出较高的组成型SAA mRNA表达。在马中，在肺、乳腺、胰腺、滑膜、胸腺、甲状腺和子宫中检测到中度（>肝表达的1%）SAA mRNA表达。所采集的其他马组织和器官包括脂肪组织、肾上腺、主动脉、脑、不同的胃肠道组织、心脏、肾脏、淋巴结、卵巢、睾丸、前列腺、骨骼肌和心肌、皮肤和脾脏；所有这些组织均显示低水平（<肝表达的1%）SAA mRNA表达。在牛中，SAA mRNA在脂肪组织、结肠、空肠、乳腺、骨骼肌、滑膜、胸腺甲状腺和子宫中中度表达；在其余样本（与马相同的组织组）中表达较低。结果证实肝脏是SAA产生的主要部位。尽管在表达SAA mRNA的组织中存在一些种间差异，但几个与外部环境相通的器官（肺、乳腺、子宫和胃肠道的某些部分）显示出SAA mRNA表达，这支持了SAA可能在抵御入侵病原体的先天防御中发挥作用的假说。因此，该研究结果值得对肝脏和肝脏外产生的SAA亚型的功能进行进一步研究。
