Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Department of Genetics and Developmental Biology, Southeast University Medical School, Nanjing 210009, China.
Environ Toxicol Pharmacol. 2009 Jul;28(1):125-32. doi: 10.1016/j.etap.2009.03.009. Epub 2009 Mar 24.
In this study, specific developmental stage for adults from day 1 to day 10 was selected to evaluate the chronic metal toxicity, because the population of dead nematodes and the accumulation of intestinal autofluorescence increased sharply after day 10. Chronic exposure to Cr, Pb, Cu, and Hg caused a significant elevation in fractions of dead animals after day 4, and resulted in a significant induction of hsp-16.2::gfp expression at all assayed metal concentrations. Moreover, chronic exposure to Ag, Cr, Pb, Cu, Hg, and Cd would induce a more severe stress response than exposure to Zn and Mn in intestine, and chronic exposure to Pb, Hg, Cr, Zn, and Mn would induce a more severe stress response than exposure to Ag, Cu and Cd in head neurons. Therefore, in determining the usefulness of animals in metal toxicity assessment, this study established a method using nematodes in testing the chronic metal toxicity.
在这项研究中，选择了从第 1 天到第 10 天的特定发育期来评估慢性金属毒性，因为在第 10 天后，死亡线虫的数量和肠道自发荧光的积累急剧增加。慢性暴露于 Cr、Pb、Cu 和 Hg 会在第 4 天后导致死亡动物的比例显著升高，并导致在所有测定的金属浓度下 hsp-16.2::gfp 表达的显著诱导。此外，与暴露于 Zn 和 Mn 相比，Ag、Cr、Pb、Cu、Hg 和 Cd 的慢性暴露会在肠道中引起更严重的应激反应，而 Pb、Hg、Cr、Zn 和 Mn 的慢性暴露会在头部神经元中引起比 Ag、Cu 和 Cd 更严重的应激反应。因此，在确定动物在金属毒性评估中的有用性方面，本研究建立了一种使用线虫测试慢性金属毒性的方法。
