Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Department of Genetics and Developmental Biology, Southeast University Medical School, Nanjing 210009, China.
Environ Toxicol Pharmacol. 2009 Nov;28(3):459-64. doi: 10.1016/j.etap.2009.07.008. Epub 2009 Aug 5.
Adaptive response to neurotoxicity on locomotion behavior by severe metal exposure was investigated in Caenorhabditis elegans. Exposure to 2.5μM of metals induced a moderate but significant reduction of locomotion behavior and induction of hsp-16.2::gfp expression. After pre-exposure to 2.5μM of metals, the reduced locomotion behavior induced by subsequent 50 and 100μM of metal exposure were significantly prevented, and the induction of hsp-16.2::gfp expression caused by subsequent 50 and 100μM of metal exposure were significantly suppressed. In contrast, after pre-exposure to 50μM examined metals, the reduced locomotion behavior induced by subsequent 50 and 100μM metal exposure were further decreased, and the noticeable induction of hsp-16.2::gfp expression caused by subsequent severe metal exposure were further enhanced. Therefore, pre-treatment with mild metal exposure can activate the adaptive response to neurotoxicity on locomotion behavior induced by subsequent severe metal exposure in nematodes.
本研究旨在探讨秀丽隐杆线虫在遭受严重金属毒性时，对神经毒性的运动行为的适应性反应。结果表明，暴露于 2.5μM 的金属会导致运动行为中度但显著降低，并诱导 hsp-16.2::gfp 的表达。在 2.5μM 的金属预暴露后，后续 50μM 和 100μM 金属暴露引起的运动行为降低明显受到抑制，而 hsp-16.2::gfp 的诱导表达也受到显著抑制。相比之下，在 50μM 金属预暴露后，后续 50μM 和 100μM 金属暴露引起的运动行为降低进一步加剧，而 hsp-16.2::gfp 的诱导表达也进一步增强。因此，轻度金属暴露预处理可以激活线虫对后续严重金属暴露引起的神经毒性的运动行为的适应性反应。
