Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan.
Parasitol Res. 2012 Feb;110(2):1025-8. doi: 10.1007/s00436-011-2564-4. Epub 2011 Jul 23.
Giardia duodenalis was found in three patients and a health care worker at a rehabilitation institution for developmentally disabled people. The four isolates were genotyped and subtyped by multilocus homology searching and phylogenetic analyses of the following four loci: glutamate dehydrogenase (gdh), triosephosphate isomerase (tpi), and β-giardin (bg) as variable loci, and elongation factor 1 alpha (ef1α) as a conserved locus. The partial sequences, gdh (709 bp), tpi (526 bp), bg (724 bp), and ef1α (680 bp) of four isolates obtained were mutually identical, and the isolates were found to be a novel subtype in sub-assemblage BIV, strongly indicating that person-to-person transmission by a single subtype occurred at the institution.
在一家发育障碍人士康复机构中，发现 3 名患者和 1 名医护人员感染了十二指肠贾第鞭毛虫。对 4 株分离株进行基因分型和亚型分析，通过多基因座同源搜索和以下 4 个基因座的系统发育分析：谷氨酸脱氢酶（gdh）、磷酸丙糖异构酶（tpi）、β-微管蛋白（bg）作为可变基因座，以及延伸因子 1α（ef1α）作为保守基因座。获得的 4 株分离株的 gdh（709bp）、tpi（526bp）、bg（724bp）和 ef1α（680bp）部分序列完全相同，这些分离株被发现属于 BIV 亚群中的一种新型亚型，强烈表明该机构内发生了单一亚型的人际传播。
