Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad 380009, Gujarat, India.
Environ Toxicol Pharmacol. 2010 Sep;30(2):103-9. doi: 10.1016/j.etap.2010.04.001. Epub 2010 May 20.
India is one of the most endemic areas, where malaria predominates and its control has become a formidable task. Chloroquine phosphate (CQ) on account of its rapid action on blood schizontocide of all the malarial parasite strains has become the most widely prescribed drug for prophylaxis and treatment of malaria. Toxicity of CQ is most commonly encountered at therapeutic and higher doses of treatment. Thus, the present study was undertaken to evaluate the protective effect of Curcumin, a herbal antioxidant obtained from Curcuma longa, on hepatic biochemical and histopathological status of CQ induced male mice. Swiss albino male mice were administered oral doses of CQ (100mg/kg body wt., 200mg/kg body wt. and 300mg/kg body wt.) and CQ+curcumin (300mg/kg body wt.+80mg/kg body wt.) for 45 days. A withdrawal of high dose treatment for 45 days was also studied. Administration of CQ brought about a significant decrease in Protein content with a decline in SDH, ATPase and ALKase activities, whereas ACPase activity was found to be significantly increased following CQ treatment. Antioxidant enzyme SOD registered a significant reduction as opposed to TBARS which was found to be elevated in a significant manner in the CQ treated groups as compared to control. Gravimetric indices (body weight and organ weight) declined significantly following CQ treatment. Administration of curcumin exhibited significant reversal of CQ induced toxicity in hepatic tissue. Protein content, SDH, ATPase, ALKase, ACPase, SOD, TBARS, body weight and organ weight were found to be comparable to that of control group after curcumin administration. Thus, obtained results led us to conclude the curative potential of curcumin against CQ induced hepatotoxicity.
印度是疟疾流行最严重的地区之一，控制疟疾已成为一项艰巨的任务。磷酸氯喹（CQ）因其对所有疟原虫血裂殖体的快速作用，已成为预防和治疗疟疾最广泛使用的药物。CQ 的毒性在治疗剂量和更高剂量时最为常见。因此，本研究旨在评估从姜黄（Curcuma longa）中获得的草药抗氧化剂姜黄素对 CQ 诱导的雄性小鼠肝生化和组织病理学状态的保护作用。给瑞士白化雄性小鼠口服 CQ（100mg/kg 体重、200mg/kg 体重和 300mg/kg 体重）和 CQ+姜黄素（300mg/kg 体重+80mg/kg 体重）45 天。还研究了停止高剂量治疗 45 天的情况。CQ 的给药导致蛋白质含量显著下降，同时 SDH、ATP 酶和 ALK 酶活性下降，而 ACP 酶活性在 CQ 处理后发现显著增加。抗氧化酶 SOD 的登记与 TBARS 相比显著降低，TBARS 在 CQ 处理组中显著升高，与对照组相比。给药 CQ 后，体重和器官重量的重量指数显著下降。姜黄素的给药表现出对 CQ 诱导的肝毒性的显著逆转。给药姜黄素后，蛋白质含量、SDH、ATP 酶、ALK 酶、ACP 酶、SOD、TBARS、体重和器官重量与对照组相比均无显著差异。因此，得出的结果使我们得出结论，姜黄素对 CQ 诱导的肝毒性具有治疗潜力。
