Department of Biotechnology and Food Engineering, Bengbu College, Bengbu 233030, PR China.
Food Chem Toxicol. 2011 Oct;49(10):2670-5. doi: 10.1016/j.fct.2011.07.020. Epub 2011 Jul 20.
Two polysaccharides, low molecular weight hyaluronic acid-1 (LMWHA-1) and LMWHA-2, with their molecular weight of 1.45×10(5) and 4.52×10(4)Da, respectively, were prepared from high molecular weight hyaluronic acid (HA,1.05×10(6)Da). LMWHA-1, LMWHA-2 and HA were studied for their antioxidant activities. In vitro antioxidant assay, LMWHA showed strong inhibition of lipid peroxidation and scavenging activities of hydroxyl radical, moderate 1,1-diphenyl-2-picryldydrazyl radical and superoxide anion scavenging activity. In addition, the LMWHA-1 exhibited much stronger antioxidant activity than LMWHA-2 and HA. For antioxidant testing in vivo, LMWHA-1, LMWHA-2 and HA were orally administrated over a period of 7days in a cyclophosphamide(CY) induced immunosuppressed mice model. As results, administration of LMWHA was able to overcome CY-induced immunosuppression and significantly raised the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant capacity (TAOC) in immunosuppressed mice. The results showed that the LMWHA, possessing pronounced free radical scavenging and antioxidant activities.
两种多糖，低分子量透明质酸-1（LMWHA-1）和 LMWHA-2，其分子量分别为 1.45×10(5)和 4.52×10(4)Da，是从高分子量透明质酸（HA，1.05×10(6)Da）中制备得到的。研究了 LMWHA-1、LMWHA-2 和 HA 的抗氧化活性。体外抗氧化试验表明，LMWHA 对脂质过氧化具有很强的抑制作用，对羟基自由基、1,1-二苯基-2-苦基肼自由基和超氧阴离子的清除能力适中。此外，LMWHA-1 表现出比 LMWHA-2 和 HA 更强的抗氧化活性。在体内抗氧化试验中，LMWHA-1、LMWHA-2 和 HA 在环磷酰胺（CY）诱导的免疫抑制小鼠模型中连续口服给药 7 天。结果表明，LMWHA 的给药能够克服 CY 诱导的免疫抑制，并显著提高免疫抑制小鼠中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和总抗氧化能力（TAOC）的活性。结果表明，LMWHA 具有显著的自由基清除和抗氧化活性。
