Department of Food Science and Technology, Jinling College, Nanjing Normal University, Nanjing 210097, China.
Carbohydr Polym. 2013 Sep 12;97(2):849-54. doi: 10.1016/j.carbpol.2013.06.024. Epub 2013 Jun 26.
Exopolysaccharide (EPS) of Lactococcus lactis subsp. lactis was isolated and purified from MRS culture broth. Phosphorylated exopolysaccharide (P-EPS) was synthesized by using the purified EPS and sodium hexametaphosphate (SHMP). The in vitro and in vivo antioxidant activity of EPS and P-EPS was analyzed. Both EPS and P-EPS displayed superoxide anion (O(2-•)), hydroxyl radical (OH•) and DPPH scavenging activity. Catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity increased in serum and the livers of mice treated with EPS and P-EPS, while malondialdehyde (MDA) levels decreased. P-EPS was shown to prevent the progression of D-galactose-induced oxidative stress in hepatocytes in vivo. P-EPS showed stronger in vitro and in vivo antioxidant activity than EPS.
从 MRS 培养液中分离和纯化乳球菌乳亚种的胞外多糖（EPS）。用纯化的 EPS 和六偏磷酸钠（SHMP）合成磷酸化胞外多糖（P-EPS）。分析 EPS 和 P-EPS 的体外和体内抗氧化活性。EPS 和 P-EPS 均表现出超氧阴离子（O(2-•)）、羟基自由基（OH•）和 DPPH 清除活性。用 EPS 和 P-EPS 处理的血清和小鼠肝脏中的过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性增加，而丙二醛（MDA）水平降低。P-EPS 可防止体内 D-半乳糖诱导的肝细胞氧化应激进展。P-EPS 的体外和体内抗氧化活性均强于 EPS。
