srd1，一种酿酒酵母中rrp1 - 1温度敏感型前体核糖体RNA加工缺陷的抑制因子。
srd1, a Saccharomyces cerevisiae suppressor of the temperature-sensitive pre-rRNA processing defect of rrp1-1.
作者信息
Fabian G R, Hess S M, Hopper A K
机构信息
Department of Biological Chemistry, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
出版信息
Genetics. 1990 Mar;124(3):497-504. doi: 10.1093/genetics/124.3.497.
Abstract
We define a new gene, SRD1, involved in the processing of pre-rRNA to mature rRNA. The SRD1 gene was identified by selecting for second-site suppressors of the previously described rrp1-1 mutation. The rrp1-1 mutation causes temperature-sensitive growth, a conditional defect in processing of 27S pre-rRNA to mature 25S rRNA, and a nonconditional increase in sensitivity to several aminoglycoside antibiotics. All srd1 alleles identified are recessive and apparently specific to the rrp1-1 mutation. Although a mutation of SRD1 suppresses the pre-rRNA processing defect, drug sensitivity and thermolethality of a point mutation of RRP1, it is unable to suppress a rrp1-disruption allele. We suggest that the SRD1 gene product either interacts with or regulates the RRP1 product.
摘要
我们定义了一个新基因SRD1，它参与前体核糖体RNA（pre-rRNA）加工成成熟核糖体RNA（rRNA）的过程。通过筛选先前描述的rrp1-1突变的第二位点抑制子，鉴定出了SRD1基因。rrp1-1突变导致温度敏感型生长、27S前体rRNA加工成成熟25S rRNA的条件性缺陷，以及对几种氨基糖苷类抗生素的敏感性无条件增加。鉴定出的所有srd1等位基因都是隐性的，且显然对rrp1-1突变具有特异性。虽然SRD1的突变抑制了RRP1点突变的前体rRNA加工缺陷、药物敏感性和热致死性，但它无法抑制rrp1破坏等位基因。我们认为SRD1基因产物要么与RRP1产物相互作用，要么对其进行调控。
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