Unité de Protéomie Fonctionnelle and Biopréservation Alimentaire, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université Tunis El Manar, Tunisie.
Evol Bioinform Online. 2011;7:99-105. doi: 10.4137/EBO.S7122. Epub 2011 Jun 28.
Triacylglycerol lipases have been thoroughly characterized in mammals and microorganisms. By contrast, very little is known about plant lipases. In this investigation, a homology model of Arabidopsis thaliana lipase (NP_179126) was constructed using a human gastric lipase (PDB ID: 1HLG), as a template for model building. This model was then assessed for stereochemical quality and side chain environment. Natural substrates: tributyrin, trioctanoin and triolen were docked into the model to investigate ligand-substrate interaction.
三酰基甘油脂肪酶在哺乳动物和微生物中得到了深入的研究。相比之下，关于植物脂肪酶的了解则非常有限。在这项研究中，使用人类胃脂肪酶（PDB ID：1HLG）作为模板，构建了拟南芥脂肪酶（NP_179126）的同源模型。然后，对该模型的立体化学质量和侧链环境进行了评估。将天然底物：丁酸三酯、辛酸三酯和三油酸酯对接入模型中，以研究配体-底物相互作用。
