Liang Yi, Wang Cun-Xin, Fang Jian-Qiao, Shao Xiao-Mei, Ying Xiao-Ming
The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Zhongguo Zhen Jiu. 2011 Dec;31(12):1101-5.
To observe anti-fatigue effect and mechanisms of pre-electroacupuncture (EA) at "Zusanli" (ST 36) in rats undergoing acute treadmill running.
Fifty male SD rats were randomly divided into three groups: a quiet group (group Q, n = 10), a model group (group M, n = 20) and an EA preconditioning group (group EAP, n = 20). After adaptation for undergoing treadmill running, all the rats in group M and group EAP were trained on acute treadmill running. Besides, EA with continuous waves, 2 Hz in frequency and 2 mA in intensity was applied at bilateral "Zusanli" (ST 36) for 30 min, which was applied once daily for continuous 6 days before treadmill running for the rats in Group EAP. Plasma lactate contents were measured immediately and 3 hours after treadmill running, respectively. Changes of dopamine (DA) and serotonin (5-HT) contents obtained immediately and 3 hours after treadmill running, respectively, in hypothalamus and striatum, were detected and compared, and DA/5-HT ratios were calculated.
Compared with group Q, the levels of blood lactate and hypothalamic 5-HT tented to increase in rats of group M, and the contents of hypothalamic DA increased significantly (P < 0.01), while the contents of striatal DA and 5-HT in group M decreased significantly (both P < 0.01) at 3 h after treadmill running. Immediately after treadmill running, the contents of DA and 5-HT increased significantly in hypothalamus (both P < 0.01), but decreased significantly in striatum (both P < 0.01) in group EAP, compared with those in group M. Moreover, EA pretreatment markedly decreased the levels of blood lactate (P < 0.05) and hypothalamic 5-HT (P < 0.01), and obviously elevated the ratio of DA/5-HT in the hypothalamus (P < 0.01) at 3 h after treadmill running.
Preventive EA at "Zusanli" (ST 36) can accelerate recovery from fatigue, which may be related to its reducing accumulation of blood lactate, elevating DA/ 5-HT ratio in the hypothalamus of the rats undergoing treadmill running.
观察预电针大鼠“足三里”（ST 36）对急性跑台运动大鼠抗疲劳的作用及机制。
50只雄性SD大鼠随机分为三组：安静组（Q组，n = 10）、模型组（M组，n = 20）和电针预处理组（EAP组，n = 20）。适应性跑台运动后，M组和EAP组大鼠进行急性跑台运动训练。此外，EAP组大鼠在跑台运动前，于双侧“足三里”（ST 36）施加频率2 Hz、强度2 mA的连续波电针30 min，每天1次，连续6天。分别于跑台运动后即刻及3小时测定血浆乳酸含量。检测并比较跑台运动后即刻及3小时下丘脑和纹状体中多巴胺（DA）和5-羟色胺（5-HT）含量的变化，并计算DA/5-HT比值。
与Q组比较，M组大鼠跑台运动后3小时血乳酸水平及下丘脑5-HT含量有升高趋势，下丘脑DA含量显著升高（P < 0.01），纹状体DA和5-HT含量显著降低（均P < 0.01）。与M组比较，EAP组大鼠跑台运动后即刻下丘脑DA和5-HT含量显著升高（均P < 0.01），纹状体DA和5-HT含量显著降低（均P < 0.01）。此外，电针预处理显著降低了跑台运动后3小时血乳酸水平（P < 0.05）和下丘脑5-HT含量（P < 0.01），明显升高了下丘脑DA/5-HT比值（P < 0.01）。
预防性电针“足三里”（ST 36）可加速疲劳恢复，其机制可能与减少血乳酸堆积、提高跑台运动大鼠下丘脑DA/5-HT比值有关。
