玉兰杂种的完整质体基因组与玉兰科物种的遗传比较。
The Complete Plastid Genome of Magnolia zenii and Genetic Comparison to Magnoliaceae species.
机构信息
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.
出版信息
Molecules. 2019 Jan 11;24(2):261. doi: 10.3390/molecules24020261.
is a critically endangered species known from only 18 trees that survive on Baohua Mountain in Jiangsu province, China. Little information is available regarding its molecular biology, with no genomic study performed on until now. We determined the complete plastid genome of and identified microsatellites. Whole sequence alignment and phylogenetic analysis using BI and ML methods were also conducted. The plastome of was 160,048 bp long with 39.2% GC content and included a pair of inverted repeats (IRs) of 26,596 bp that separated a large single-copy (LSC) region of 88,098 bp and a small single-copy (SSC) region of 18,757 bp. One hundred thirty genes were identified, of which 79 were protein-coding genes, 37 were transfer RNAs, and eight were ribosomal RNAs. Thirty seven simple sequence repeats (SSRs) were also identified. Comparative analyses of genome structure and sequence data of closely-related species revealed five mutation hotspots, useful for future phylogenetic research. was placed as sister to with strong support in all analyses. Overall, this study providing genomic resources will be beneficial for the evolutionary study and phylogenetic reconstruction of Magnoliaceae.
宝华玉兰是极危物种，仅在中国江苏省宝华山存活 18 株。关于其分子生物学的信息很少，到目前为止还没有对 进行基因组研究。我们测定了 的完整叶绿体基因组，并鉴定了微卫星。还使用 BI 和 ML 方法进行了全序列比对和系统发育分析。 的叶绿体基因组长 160048bp，GC 含量为 39.2%，包含一对长 26596bp 的反向重复（IRs），它们分隔了长 88098bp 的大单拷贝（LSC）区和长 18757bp 的小单拷贝（SSC）区。共鉴定到 130 个基因，其中 79 个是蛋白质编码基因，37 个是转移 RNA，8 个是核糖体 RNA。还鉴定到 37 个简单序列重复（SSR）。与近缘物种的基因组结构和序列数据的比较分析揭示了五个突变热点，这对未来的系统发育研究很有用。在所有分析中， 都被强烈支持为玉兰的姐妹种。总的来说，这项研究为 提供了基因组资源，将有利于木兰科的进化研究和系统发育重建。
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