同卵双胞胎中慢性疲劳综合征不一致的嗜异性鼠白血病病毒相关病毒。
Xenotropic murine leukemia virus-related virus in monozygotic twins discordant for chronic fatigue syndrome.
机构信息
Department of Laboratory Medicine, University of Washington, Seattle, WA 98105, USA.
出版信息
Diagn Microbiol Infect Dis. 2011 Sep;71(1):66-71. doi: 10.1016/j.diagmicrobio.2011.06.003. Epub 2011 Jul 26.
A recent report suggested an association between xenotropic murine leukemia virus-related virus (XMRV) and chronic fatigue syndrome (CFS). If confirmed, this would suggest that antiretroviral therapy might benefit patients suffering from CFS. We validated a set of assays for XMRV and evaluated the prevalence of XMRV in a cohort of monozygotic twins discordant for CFS. Stored peripheral blood mononuclear cell (PBMC) samples were tested with 3 separate polymerase chain reaction (PCR) assays (one of which was nested) for XMRV DNA, and serum/plasma was tested for XMRV RNA by reverse transcription (RT)-PCR. None of the PBMC samples from the twins with CFS or their unaffected co-twins was positive for XMRV, by any of the assays. One plasma sample, from an unaffected co-twin, was reproducibly positive by RT-PCR. However, serum from the same day was negative, as was a follow-up plasma sample obtained 2 days after the positive specimen. These data do not support an association of XMRV with CFS.
最近的一份报告表明，嗜异性鼠白血病病毒相关病毒（XMRV）与慢性疲劳综合征（CFS）之间存在关联。如果得到证实，这将表明抗逆转录病毒疗法可能有益于患有 CFS 的患者。我们验证了一组用于 XMRV 的检测方法，并评估了一组同卵双胞胎中 XMRV 的患病率，这些双胞胎中 CFS 不一致。使用 3 种不同的聚合酶链反应（PCR）检测方法（其中一种为嵌套）对 XMRV DNA 进行了检测，并用逆转录（RT）-PCR 对血清/血浆中的 XMRV RNA 进行了检测。通过任何一种检测方法，患有 CFS 的双胞胎及其未受影响的同卵双胞胎的 PBMC 样本均未呈 XMRV 阳性。一份来自未受影响的同卵双胞胎的血浆样本通过 RT-PCR 可重复呈阳性。然而，同一天的血清为阴性，阳性样本两天后获得的后续血浆样本也为阴性。这些数据不支持 XMRV 与 CFS 之间存在关联。
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