Department of Pathology, University of Washington, Seattle WA, USA.
Retrovirology. 2010 Dec 20;7:112. doi: 10.1186/1742-4690-7-112.
Efforts to assess the prevalence of xenotropic murine leukemia virus-related virus (XMRV) in patients with prostate cancer and chronic fatigue syndrome have relied heavily on PCR-based testing of clinical samples and have yielded widely divergent findings. This week in Retrovirology, reports from four independent research groups illustrate the extreme care needed to exclude DNA or RNA contamination in PCR analyses of XMRV. In addition, phylogenetic evidence suggesting that previously-published XMRV sequences originated from a commonly-used prostate carcinoma cell line (22Rv1) is presented. These findings raise important questions regarding the provenance of XMRV and its potential connection to human disease.
评估前列腺癌和慢性疲劳综合征患者中嗜性鼠白血病病毒相关病毒（XMRV）流行率的努力主要依赖于临床样本的基于 PCR 的检测，并且产生了广泛不同的结果。本周在 Retrovirology 杂志上，来自四个独立研究小组的报告说明了在 XMRV 的 PCR 分析中排除 DNA 或 RNA 污染所需的极端谨慎。此外，还提出了进化证据表明，以前发表的 XMRV 序列源自常用的前列腺癌细胞系（22Rv1）。这些发现提出了有关 XMRV 的起源及其与人类疾病潜在联系的重要问题。
