University of Tsukuba, Graduate School of Comprehensive Human Sciences, 1-1-1 Tennodai, Tsukuba 305-8577, Japan.
Neurosci Res. 2011 Nov;71(3):311-4. doi: 10.1016/j.neures.2011.07.1824. Epub 2011 Jul 23.
Dendritic spines are postsynaptic structures which are formed from filopodia. We examined roles of serotonin (5-HT) receptors in the spine formation. Embryonic rat cortical neurons were cultured for 10 or 14 days and treated by 5-HT receptor agonists for 24 h. At 11 days in vitro, 5-HT(1A) agonist increased filopodia density, whereas 5-HT(2A/2C) agonist increased the density of puncta and spines. At 15 days in vitro, 5-HT(1A) agonist decreased the density of puncta and spines, whereas 5-HT(2A/2C) agonist decreased filopodia density with increase of spines. In conclusion, the present study shows 5-HT receptors have subtype-specific effects on the spine formation.
树突棘是由丝状伪足形成的突触后结构。我们研究了 5-羟色胺（5-HT）受体在棘突形成中的作用。培养胚胎大鼠皮质神经元 10 或 14 天，并通过 5-HT 受体激动剂处理 24 小时。在体外 11 天，5-HT（1A）激动剂增加丝状伪足密度，而 5-HT（2A/2C）激动剂增加点状和棘突密度。在体外 15 天，5-HT（1A）激动剂降低点状和棘突密度，而 5-HT（2A/2C）激动剂降低丝状伪足密度，同时增加棘突密度。总之，本研究表明 5-HT 受体对棘突形成具有亚型特异性作用。
