Department of Rickettsiology and Anaplasmosis, National Institute of Communicable Disease Control and Prevention, China CDC, Beijing 102206, China.
Asian Pac J Trop Med. 2011 Jul;4(7):568-72. doi: 10.1016/S1995-7645(11)60148-X.
To investigate the situation of anaplasmosis in Yiyuan county, Shandong Province.
A total of 26 blood samples from febrile patients suspected of anaplasmosis, 48 blood samples from healthy farmers, 8 from dogs, and 10 from goats and 170 ticks were collected in the same area during 2005-2007, and detected by serological and molecular methods.
Eight confirmed cases and 6 probable cases were determined using serologic and molecular methods. The seroprevalence of Anaplasma phagocytophilum (A. phagocytophilum) was 26.7% in healthy cases. Nine out of 10 sheep samples and 7 out of 8 dog samples reacted positively to the A. phagocytophilum antigen. PCR amplification and sequencing of the 16SrRNA of A. phagocytophilum gene showed that some samples from patients, goats and ticks were 100% identical. The seroprevalence of Rickettsia typhi was 22.9%, Orientia tsutsugamushi 6.3%, Rickettsia sibirica 27.1%, Coxiella burnetii 18.8%, Bartonella henselae 31.3%, and Borrelia burgdorferi 41.6%.
It is important to make differential diagnosis of febrile patients and to apply treatment with specific antibiotics. It is needed to enforce essential prevention and control measures including tick control and to improve sanitation conditions.
调查山东省沂源县无形体病的流行情况。
2005-2007 年在同一地区共采集疑似无形体病发热患者 26 份血样、健康农民 48 份血样、犬 8 份血样、羊 10 份血样和蜱 170 只，采用血清学和分子生物学方法进行检测。
血清学和分子生物学方法共确诊 8 例确认病例和 6 例疑似病例。健康人群中无形体血清阳性率为 26.7%。10 只羊中有 9 只、8 只犬中有 7 只的样品对无形体抗原呈阳性反应。对无形体 16SrRNA 基因的 PCR 扩增和测序显示，患者、羊和蜱的部分样本完全相同。伤寒立克次体的血清阳性率为 22.9%，恙虫病东方体为 6.3%，西伯利亚立克次体为 27.1%，伯氏考克斯体为 18.8%，汉赛巴通体为 31.3%，伯氏疏螺旋体为 41.6%。
对发热患者进行鉴别诊断并应用特效抗生素进行治疗非常重要。需要采取蜱控制等基本的防控措施，并改善卫生条件。
