Institute for Environmental Science and Technology, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama 338-8570, Japan.
Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13835-40. doi: 10.1073/pnas.1104809108. Epub 2011 Jul 29.
Nonphotochemical quenching (NPQ) regulates energy conversion in photosystem II and protects plants from photoinhibition. Here we analyze NPQ capacity in a number of rice cultivars. NPQ was strongly induced under medium and high light intensities in rice leaves. Japonica cultivars generally showed higher NPQ capacities than Indica cultivars when we measured a rice core collection. We mapped NPQ regulator and identified a locus (qNPQ1-2) that seems to be responsible for the difference in NPQ capacity between Indica and Japonica. One of the two rice PsbS homologues (OsPsbS1) was found within the qNPQ1-2 region. PsbS protein was not accumulated in the leaf blade of the mutant harboring transferred DNA insertion in OsPsbS1. NPQ capacity increased as OsPsbS1 expression increased in a series of transgenic lines ectopically expressing OsPsbS1 in an Indica cultivar. Indica cultivars lack a 2.7-kb region at the point 0.4 kb upstream of the OsPsbS1 gene, suggesting evolutionary discrimination of this gene.
非光化学猝灭（NPQ）调节光合作用系统 II 的能量转换，并保护植物免受光抑制。在这里，我们分析了许多水稻品种的 NPQ 能力。在中光和高光强下，水稻叶片中的 NPQ 被强烈诱导。当我们测量水稻核心收集时，粳稻品种通常比籼稻品种表现出更高的 NPQ 能力。我们对 NPQ 调节剂进行了作图，并鉴定了一个似乎负责籼稻和粳稻之间 NPQ 能力差异的位点（qNPQ1-2）。两个水稻 PsbS 同源物（OsPsbS1）之一位于 qNPQ1-2 区域内。在含有 OsPsbS1 转基因插入的突变体叶片中未积累 PsbS 蛋白。在一系列转 OsPsbS1 的籼稻品系中，随着 OsPsbS1 表达的增加，NPQ 能力增加。籼稻品种在 OsPsbS1 基因上游 0.4kb 处缺失了 2.7kb 的区域，这表明该基因在进化上受到了歧视。
