蒙特卡罗荧光显微断层成像技术。
Monte Carlo fluorescence microtomography.
出版信息
J Biomed Opt. 2011 Jul;16(7):070501. doi: 10.1117/1.3596171.
Abstract
Fluorescence microscopy allows real-time monitoring of optical molecular probes for disease characterization, drug development, and tissue regeneration. However, when a biological sample is thicker than 1 mm, intense scattering of light would significantly degrade the spatial resolution of fluorescence microscopy. In this paper, we develop a fluorescence microtomography technique that utilizes the Monte Carlo method to image fluorescence reporters in thick biological samples. This approach is based on an l(0)-regularized tomography model and provides an excellent solution. Our studies on biomimetic tissue scaffolds have demonstrated that the proposed approach is capable of localizing and quantifying the distribution of optical molecular probe accurately and reliably.
摘要
荧光显微镜可以实时监测用于疾病特征描述、药物开发和组织再生的光学分子探针。然而，当生物样本厚度超过 1 毫米时，强烈的光散射会显著降低荧光显微镜的空间分辨率。在本文中，我们开发了一种荧光断层扫描技术，该技术利用蒙特卡罗方法对厚生物样本中的荧光报告分子进行成像。该方法基于 l(0)正则化断层扫描模型，提供了一种优秀的解决方案。我们在仿生组织支架上的研究表明，所提出的方法能够准确可靠地定位和量化光学分子探针的分布。
相似文献
1
Monte Carlo fluorescence microtomography.蒙特卡罗荧光显微断层成像技术。
J Biomed Opt. 2011 Jul;16(7):070501. doi: 10.1117/1.3596171.
2
Comparison of simplified Monte Carlo simulation and diffusion approximation for the fluorescence signal from phantoms with typical mouse tissue optical properties.具有典型小鼠组织光学特性的体模荧光信号的简化蒙特卡罗模拟与扩散近似的比较。
Appl Opt. 2007 Apr 1;46(10):1686-92. doi: 10.1364/ao.46.001686.
3
Fast Monte Carlo simulations of ultrasound-modulated light using a graphics processing unit.利用图形处理单元实现超声调制光的快速蒙特卡罗模拟。
J Biomed Opt. 2010 Sep-Oct;15(5):055007. doi: 10.1117/1.3495729.
4
Monte Carlo simulation of time-dependent, transport-limited fluorescent boundary measurements in frequency domain.频域中随时间变化、受传输限制的荧光边界测量的蒙特卡罗模拟
Med Phys. 2007 Apr;34(4):1298-311. doi: 10.1118/1.2710549.
5
Early-photon fluorescence tomography of a heterogeneous mouse model with the telegraph equation.基于电报方程的异质小鼠模型早期光子荧光断层成像
Appl Opt. 2011 Oct 1;50(28):5397-407. doi: 10.1364/AO.50.005397.
6
Mesh-based Monte Carlo method in time-domain widefield fluorescence molecular tomography.基于网格的时域广角荧光分子层析的蒙特卡罗方法。
J Biomed Opt. 2012 Oct;17(10):106009. doi: 10.1117/1.JBO.17.10.106009.
7
Fluorescence-lifetime-based tomography for turbid media.用于浑浊介质的基于荧光寿命的层析成像技术。
Opt Lett. 2005 Dec 15;30(24):3347-9. doi: 10.1364/ol.30.003347.
8
Fluorescence lifetime imaging from time resolved measurements using a shape-based approach.基于形状的方法从时间分辨测量中进行荧光寿命成像。
Opt Express. 2009 May 25;17(11):8843-55. doi: 10.1364/oe.17.008843.
9
Adaptive regularized method based on homotopy for sparse fluorescence tomography.基于同伦的自适应正则化方法用于稀疏荧光层析成像。
Appl Opt. 2013 Apr 10;52(11):2374-84. doi: 10.1364/AO.52.002374.
10
Optimal sparse solution for fluorescent diffuse optical tomography: theory and phantom experimental results.荧光漫射光学层析成像的最优稀疏解：理论与体模实验结果
Appl Opt. 2007 Apr 1;46(10):1679-85. doi: 10.1364/ao.46.001679.
引用本文的文献
1
Monte Carlo Simulations in Nanomedicine: Advancing Cancer Imaging and Therapy.纳米医学中的蒙特卡洛模拟：推动癌症成像与治疗
Nanomaterials (Basel). 2025 Jan 15;15(2):117. doi: 10.3390/nano15020117.
2
Recent methodology advances in fluorescence molecular tomography.荧光分子断层成像的最新方法进展
Vis Comput Ind Biomed Art. 2018 Sep 5;1(1):1. doi: 10.1186/s42492-018-0001-6.
3
A fiber-optic-based imaging system for nondestructive assessment of cell-seeded tissue-engineered scaffolds.基于光纤的成像系统，用于对细胞接种的组织工程支架进行无损评估。
Tissue Eng Part C Methods. 2012 Sep;18(9):677-87. doi: 10.1089/ten.TEC.2011.0490. Epub 2012 May 10.
本文引用的文献
1
Fabrication and mechanical characterization of 3D electrospun scaffolds for tissue engineering.用于组织工程的 3D 电纺支架的制造和机械特性。
Biomed Mater. 2010 Oct;5(5):055006. doi: 10.1088/1748-6041/5/5/055006. Epub 2010 Sep 15.
2
Multilevel bioluminescence tomography based on radiative transfer equation Part 1: l1 regularization.基于辐射传输方程的多层生物发光断层成像 第1部分：l1正则化
Opt Express. 2010 Feb 1;18(3):1854-71. doi: 10.1364/OE.18.001854.
3
Differential evolution approach for regularized bioluminescence tomography.正则化生物发光断层成像的差分进化算法。
IEEE Trans Biomed Eng. 2010 Sep;57(9):2229-38. doi: 10.1109/TBME.2010.2041452. Epub 2010 Feb 17.
4
Quantifying cellular interaction dynamics in 3D fluorescence microscopy data.量化三维荧光显微镜数据中的细胞相互作用动态
Nat Protoc. 2009;4(9):1305-11. doi: 10.1038/nprot.2009.129. Epub 2009 Aug 20.
5
Practical reconstruction method for bioluminescence tomography.生物发光断层成像的实用重建方法。
Opt Express. 2005 Sep 5;13(18):6756-71. doi: 10.1364/opex.13.006756.
6
Source reconstruction for spectrally-resolved bioluminescence tomography with sparse a priori information.基于稀疏先验信息的光谱分辨生物发光断层成像源重建
Opt Express. 2009 May 11;17(10):8062-80. doi: 10.1364/oe.17.008062.
7
Parallel computing with graphics processing units for high-speed Monte Carlo simulation of photon migration.利用图形处理单元进行并行计算以实现光子迁移的高速蒙特卡罗模拟。
J Biomed Opt. 2008 Nov-Dec;13(6):060504. doi: 10.1117/1.3041496.
8
In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography.利用介观荧光断层扫描对黑腹果蝇蛹进行体内成像。
Nat Methods. 2008 Jan;5(1):45-7. doi: 10.1038/nmeth1149. Epub 2007 Dec 9.
9
Fluorescence microscopy.荧光显微镜术
Nat Methods. 2005 Dec;2(12):910-9. doi: 10.1038/nmeth817.
10
Uniqueness theorems in bioluminescence tomography.生物发光断层成像中的唯一性定理
Med Phys. 2004 Aug;31(8):2289-99. doi: 10.1118/1.1766420.
Zotero 插件