Department of Metabolic Care and Gerontology, University Hospital, Hradec Kralove, Czech Republic.
Talanta. 2011 Sep 15;85(3):1466-71. doi: 10.1016/j.talanta.2011.06.027. Epub 2011 Jun 29.
A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithic cartridge 4.6 mm × 50 mm+3.0 mm × 100 mm and 4.6 × 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice.
建立并验证了一种新的高效液相色谱法，用于同时测定人血清中的新蝶呤、肌酐、犬尿氨酸和色氨酸。整体式固定相技术（两根整体式 RP-18e 柱通过保护整体式柱芯 4.6mm×50mm+3.0mm×100mm 和 4.6×10mm 相连接）和用于微量滴定板的特殊自动进样器（样品避光保存在冷却的地方，以防止蒸发）与简单的样品制备步骤相结合。采用 15mmol/L 磷酸盐缓冲液（pH4.50）作为流动相。新蝶呤和色氨酸用荧光检测法检测，犬尿氨酸和肌酐用二极管阵列检测法检测。该方法适用于临床研究和常规实践中的大量样本序列。
