基于“开-关”模式的荧光 Cu2+ 化学传感器在水相介质和活细胞中的应用。
"Off-On" based fluorescent chemosensor for Cu2+ in aqueous media and living cells.
机构信息
Key Laboratory of Coastal Zone Environmental Processes, CAS, Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China.
出版信息
Talanta. 2011 Sep 15;85(3):1627-33. doi: 10.1016/j.talanta.2011.06.057. Epub 2011 Jun 29.
A novel Cu(2+)-specific "off-on" fluorescent chemosensor of naphthalimide modified rhodamine B (naphthalimide modified rhodamine B chemosensor, NRC) was designed and synthesized, based on the equilibrium between the spirolactam (non-fluorescence) and the ring-opened amide (fluorescence). The chemosensor NRC showed high Cu(2+)-selective fluorescence enhancement over commonly coexistent metal ions or anions in neutral aqueous media. The limit of detection (LOD) based on 3×δ(blank)/k was obtained as low as 0.18 μM of Cu(2+), as well as an excellent linearity of 0.05-4.5 μM (R=0.999), indicating the chemosensor of high sensitivity and wide quantitation range. And also the coordination mode with 1:1 stoichiometry was proposed between NRC and Cu(2+). In addition, the effects of pH, co-existing metal ions and anions, and the reversibility were investigated in detail. It was also demonstrated that the NRC could be used as an excellent "off-on" fluorescent chemosensor for the measurement of Cu(2+) in living cells with satisfying results, which further displayed its valuable applications in biological systems.
一种基于萘酰亚胺修饰的罗丹明 B（萘酰亚胺修饰的罗丹明 B 化学传感器，NRC）的新型 Cu(2+)特异性“开-关”荧光化学传感器被设计和合成，基于螺环内酰胺（非荧光）和开环酰胺（荧光）之间的平衡。在中性水介质中，化学传感器 NRC 对常见共存的金属离子或阴离子表现出高选择性的 Cu(2+)荧光增强。基于 3×δ(空白)/k 的检测限（LOD）低至 0.18 μM 的 Cu(2+)，以及 0.05-4.5 μM（R=0.999）的优异线性度，表明该化学传感器具有高灵敏度和宽定量范围。并且还提出了 NRC 与 Cu(2+)之间的 1:1 化学计量比的配位模式。此外，详细研究了 pH、共存金属离子和阴离子以及可逆性的影响。还证明，NRC 可以用作活细胞中 Cu(2+)测量的出色“开-关”荧光化学传感器，结果令人满意，进一步显示了其在生物系统中的有价值应用。
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