Dobbie Ian M, King Emma, Parton Richard M, Carlton Peter M, Sedat John W, Swedlow Jason R, Davis Ilan
Cold Spring Harb Protoc. 2011 Aug 1;2011(8):899-909. doi: 10.1101/pdb.top121.
Bioimaging is currently undergoing an exciting revolution. This includes all aspects of imaging from probe development, specimen preparation, and instrumentation to image analysis and quantitation. Perhaps the most exciting developments are new platforms for imaging that radically advance the capabilities for collecting high spatial and temporal resolution data. In many cases, the standard microscope has been replaced with new purpose-built platforms that are much more flexible, and enable the implementation of new imaging modalities. such as particular single molecule and superresolution imaging methods.
生物成像目前正在经历一场令人兴奋的变革。这包括从探针开发、样本制备、仪器设备到图像分析和定量的成像各个方面。也许最令人兴奋的进展是新的成像平台，这些平台极大地提升了收集高空间和时间分辨率数据的能力。在许多情况下，标准显微镜已被新的专用平台所取代，这些平台更加灵活，并能够实现新的成像模式，例如特定的单分子和超分辨率成像方法。
