Surface Biology Group, Department of Prosthodontics, Faculty of Odontology, Malmö University, Sweden.
Acta Biomater. 2011 Dec;7(12):4229-34. doi: 10.1016/j.actbio.2011.07.017. Epub 2011 Jul 21.
Nanostructures on implant surfaces have been shown to enhance osseointegration; however, commonly used evaluation techniques are probably not sufficiently sensitive to fully determine the effects of this process. This study aimed to observe the osseointegration properties of nanostructured calcium phosphate (CaP)-coated implants, by using a combination of three-dimensional imaging and conventional histology. Titanium implants were coated with stable CaP nanoparticles using an immersion technique followed by heat treatment. Uncoated implants were used as the control. After topographical and chemical characterizations, implants were inserted into the rabbit femur. After 2 and 4weeks, the samples were retrieved for micro-computed tomography and histomorphometric evaluation. Scanning electron microscopy evaluation indicated that the implant surface was modified at the nanoscale by CaP to obtain surface textured with rod-shaped structures. Relative to the control, the bone-to-implant contact for the CaP-coated implant was significantly higher at 4weeks after the implant surgery. Further, corresponding 3-D images showed active bone formation surrounding the implant. 3-D quantification and 2-D histology demonstrated statistical correlation; moreover, 3-D quantification indicated a statistical decrease in bone density in the non-coated control implant group between 2 and 4weeks after the surgery. The application of 3-D evaluation further clarified the temporal characteristics and biological reaction of implants in bone.
在种植体表面形成纳米结构已被证明可以增强骨整合；然而，常用的评估技术可能不够敏感，无法完全确定这一过程的效果。本研究旨在通过三维成像和常规组织学相结合的方法，观察纳米结构磷酸钙（CaP）涂层种植体的骨整合特性。使用浸渍技术和热处理在钛种植体表面涂覆稳定的 CaP 纳米颗粒。未涂层的种植体用作对照。在进行形貌和化学特性分析后，将种植体插入兔股骨中。在 2 周和 4 周后，取出样品进行微计算机断层扫描和组织形态计量评估。扫描电子显微镜评估表明，CaP 对种植体表面进行了纳米级修饰，从而获得了具有棒状结构的表面纹理。与对照组相比，在种植手术后 4 周时，CaP 涂层种植体的骨与种植体接触明显更高。此外，相应的三维图像显示了种植体周围活跃的骨形成。三维定量和二维组织学显示出统计学相关性；此外，三维定量分析表明，在手术后 2 周至 4 周期间，非涂层对照组种植体的骨密度呈统计学下降。三维评估的应用进一步阐明了植入物在骨中的时间特征和生物学反应。
