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基于金纳米粒子的表面增强拉曼散射用于胚胎干细胞分化的无创分子探测。

Gold nanoparticle-based surface-enhanced Raman scattering for noninvasive molecular probing of embryonic stem cell differentiation.

机构信息

Department of Applied Physics, Graduate School of Engineering, Osaka University, Suita City, Osaka, Japan.

出版信息

PLoS One. 2011;6(8):e22802. doi: 10.1371/journal.pone.0022802. Epub 2011 Aug 4.

DOI:10.1371/journal.pone.0022802
PMID:21829653
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3150363/
Abstract

This study reports the use of gold nanoparticle-based surface-enhanced Raman scattering (SERS) for probing the differentiation of mouse embryonic stem (mES) cells, including undifferentiated single cells, embryoid bodies (EBs), and terminally differentiated cardiomyocytes. Gold nanoparticles (GNPs) were successfully delivered into all 3 mES cell differentiation stages without affecting cell viability or proliferation. Transmission electron microscopy (TEM) confirmed the localization of GNPs inside the following cell organelles: mitochondria, secondary lysosome, and endoplasmic reticulum. Using bright- and dark-field imaging, the bright scattering of GNPs and nanoaggregates in all 3 ES cell differentiation stages could be visualized. EB (an early differentiation stage) and terminally differentiated cardiomyocytes both showed SERS peaks specific to metabolic activity in the mitochondria and to protein translation (amide I, amide II, and amide III peaks). These peaks have been rarely identified in undifferentiated single ES cells. Spatiotemporal changes observed in the SERS spectra from terminally differentiated cardiomyocyte tissues revealed local and dynamic molecular interactions as well as transformations during ES cell differentiation.

摘要

本研究报告了基于金纳米粒子的表面增强拉曼散射（SERS）在探测小鼠胚胎干细胞（mES 细胞）分化中的应用，包括未分化的单细胞、类胚体（EB）和终末分化的心肌细胞。金纳米粒子（GNPs）成功地递送至所有 3 个 mES 细胞分化阶段，而不影响细胞活力或增殖。透射电子显微镜（TEM）证实了 GNPs 定位于以下细胞细胞器内：线粒体、次级溶酶体和内质网。使用明场和暗场成像，可以观察到所有 3 个 ES 细胞分化阶段中 GNPs 和纳米聚集体的明亮散射。EB（早期分化阶段）和终末分化的心肌细胞都显示出与线粒体代谢活性和蛋白质翻译（酰胺 I、酰胺 II 和酰胺 III 峰）相关的特异性 SERS 峰。这些峰在未分化的单细胞 ES 细胞中很少被识别。终末分化的心肌细胞组织的 SERS 光谱中观察到的时空变化揭示了 ES 细胞分化过程中局部和动态的分子相互作用以及转化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/967c/3150363/6610f976a9a4/pone.0022802.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/967c/3150363/e08103597cfb/pone.0022802.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/967c/3150363/4528f17cdff9/pone.0022802.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/967c/3150363/668971332825/pone.0022802.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/967c/3150363/52a29c7db8a5/pone.0022802.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/967c/3150363/c37a22e022bc/pone.0022802.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/967c/3150363/6610f976a9a4/pone.0022802.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/967c/3150363/e08103597cfb/pone.0022802.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/967c/3150363/4528f17cdff9/pone.0022802.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/967c/3150363/668971332825/pone.0022802.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/967c/3150363/52a29c7db8a5/pone.0022802.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/967c/3150363/c37a22e022bc/pone.0022802.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/967c/3150363/6610f976a9a4/pone.0022802.g006.jpg

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