Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, PA, USA.
Cancer Biol Ther. 2011 Oct 15;12(8):737-41. doi: 10.4161/cbt.12.8.16442.
To mimic advanced stage of cancer development involving multi-organ metastasis, hydrodynamic delivery commonly used in gene transfer was explored for establishing concurrent tumors in the lung, liver and kidney using B16-F1 melanoma cells, 4T1 breast cells and Renca renal carcinoma cells, as a model. The procedure involves a rapid injection into a mouse tail-vein of serum-free medium, containing tumor cells in a volume equal to approximately 7-9% of body weight. Compared to the conventional tail vein injection of tumor cells resulting in tumor growth only in the lung, hydrodynamic injection is highly effective in establishing tumor growth in the liver, kidney and lung. All tumor cells examined including melanoma, breast metastatic, and renal carcinoma cells showed significant tumor growth in these organs. These results suggest that the hydrodynamic delivery can be a valuable tool for modeling cancer in laboratory animals, especially in experimental mice.
为了模拟涉及多器官转移的癌症晚期发展，研究人员探索了使用水动力递送技术，将 B16-F1 黑色素瘤细胞、4T1 乳腺癌细胞和 Renca 肾癌细胞作为模型，在肺部、肝脏和肾脏中同时建立肿瘤。该方法包括将无血清培养基快速注入小鼠尾静脉，其中含有相当于大约 7-9%体重的肿瘤细胞。与传统的尾静脉注射肿瘤细胞仅导致肺部肿瘤生长相比，水动力注射在肝脏、肾脏和肺部建立肿瘤生长方面非常有效。所有检查的肿瘤细胞，包括黑色素瘤、乳腺癌转移瘤和肾癌细胞，在这些器官中均显示出明显的肿瘤生长。这些结果表明，水动力递送可以成为在实验动物中模拟癌症的有价值的工具，特别是在实验小鼠中。
