Université Paris Est (UPEC), Faculté de médecine de Créteil, Créteil, France.
J Clin Pathol. 2011 Dec;64(12):1123-7. doi: 10.1136/jcp.2010.084285. Epub 2011 Aug 11.
Evaluation of the Iris iQ(®)200 Elite analyser, initially designed for urinary cell counting, for the analysis of biological fluids (serous effusion fluids and cerebrospinal drainage fluids) and comparison of its performance with that of the manual microscopic method.
Routine samples (ascite fluids, pleural fluids and cerebrospinal fluids) were evaluated in terms of red blood cells and nucleated elements using the iQ(®)200 analyser and the manual method. The authors compared the reliability, repeatability and speed of the two techniques. In addition, the authors assessed the contribution of two different sample dilution processes to the improvement of iQ(®)200 analyser cytological results.
Very good agreements were found between the two methods and between the two sample dilution processes. Regarding the repeatability, the coefficients of variation obtained with the iQ200 were slightly higher than those obtained by the manual method. Besides, the difference in the speed of the two methods was not significantly different for series with <10 samples.
The Iris iQ(®)200 Elite analyser has allowed us to obtain reliable results, equivalent to that of the manual method, for cell enumeration in biological fluids. Although the speed of this instrument needs to be improved for larger series of samples, it enables standardised and objective cytological results to be obtained and represents an alternative to the usual manual microscopic method. Moreover, automation of such analyses permits saving of technician time.
评估最初设计用于尿液细胞计数的 Iris iQ(®)200 Elite 分析仪，用于分析生物体液（浆膜腔积液和脑脊髓液），并将其性能与手动显微镜方法进行比较。
使用 iQ(®)200 分析仪和手动方法对常规样本（腹水、胸水和脑脊液）中的红细胞和有核细胞进行评估。作者比较了两种技术的可靠性、可重复性和速度。此外，作者评估了两种不同的样本稀释过程对改善 iQ(®)200 分析仪细胞学结果的贡献。
两种方法之间以及两种样本稀释过程之间都发现了非常好的一致性。关于可重复性，iQ200 获得的变异系数略高于手动方法获得的变异系数。此外，对于 <10 个样本的系列，两种方法的速度差异没有显著差异。
Iris iQ(®)200 Elite 分析仪使我们能够获得可靠的结果，与手动方法相当，用于生物体液中的细胞计数。尽管该仪器的速度需要在更大的样本系列中进行改进，但它能够获得标准化和客观的细胞学结果，并代表了对常用手动显微镜方法的替代。此外，此类分析的自动化可以节省技术人员的时间。
