Department of Chemistry, Institute of General and Ecological Chemistry, Technical University of Lodz, Zeromskiego 116, 90-924 Lodz, Poland.
Nutr Res. 2011 Jul;31(7):497-502. doi: 10.1016/j.nutres.2011.06.002.
Urinary dicarboxylic acids are an important source of information about metabolism and potential problems especially connected with energy production, intestinal dysbiosis, and nutritional individuality in autistic children. A diet rich in vitamins and macroelements is a new idea of intervention in autism. The objective of the present study was to test the hypothesis that vitamin B2, vitamin B6, and magnesium supplementation is effective in reducing the level of dicarboxylic acids in the urine of autistic children. We examined the levels of succinic, adipic, and suberic acids in the urine of autistic children before and after vitamin supplementation. Thirty children with autism received magnesium (daily dose, 200 mg), vitamin B6 (pyridoxine; daily dose, 500 mg), and vitamin B2 (riboflavin; daily dose, 20 mg). The treatment was provided for a period of 3 months. Organic acids were determined using gas chromatography/mass spectrometry. Before supplementation, the levels of succinic, adipic, and suberic acids in the urine of autistic children were 41.47 ± 50.40 μmol/mmol creatinine, 15.61 ± 15.31 μmol/mmol creatinine, 8.02 ± 6.08 μmol/mmol creatinine; and after supplementation, the levels were 9.90 ± 8.26 μmol/mmol creatinine, 2.92 ± 2.41 μmol/mmol creatinine, and 2.57 ± 3.53 μmol/mmol creatinine, respectively. The results suggest that the supplementation reduces the level of dicarboxylic acid in the urine of autistic children.
尿液中的二羧酸是了解代谢和潜在问题的重要信息来源，尤其是与自闭症儿童的能量产生、肠道菌群失调和营养个体差异有关的问题。富含维生素和大量元素的饮食是自闭症干预的新理念。本研究旨在检验以下假设，即补充维生素 B2、维生素 B6 和镁可有效降低自闭症儿童尿液中二羧酸的水平。我们检测了自闭症儿童在补充维生素前后尿液中琥珀酸、己二酸和辛二酸的水平。30 名自闭症儿童接受了镁（每日剂量 200mg）、维生素 B6（吡哆醇；每日剂量 500mg）和维生素 B2（核黄素；每日剂量 20mg）的补充。治疗持续 3 个月。使用气相色谱/质谱法测定有机酸。在补充之前，自闭症儿童尿液中的琥珀酸、己二酸和辛二酸的水平分别为 41.47 ± 50.40 μmol/mmol 肌酐、15.61 ± 15.31 μmol/mmol 肌酐和 8.02 ± 6.08 μmol/mmol 肌酐；补充后，水平分别为 9.90 ± 8.26 μmol/mmol 肌酐、2.92 ± 2.41 μmol/mmol 肌酐和 2.57 ± 3.53 μmol/mmol 肌酐。结果表明，补充可降低自闭症儿童尿液中二羧酸的水平。
