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Immunofluorescence localization of the regulatory subunit type II of cAMP-dependent protein kinase in PC12 and 3T3 cells in different proliferative states.

作者信息

Shmyrev I I, Grozdova I D, Kondratyev A D, Mamayeva E G, Severin E S

机构信息

Institute of Applied Molecular Biology, USSR Ministry of Health, Moscow.

出版信息

Mol Cell Biochem. 1990 Mar 5;93(1):47-52. doi: 10.1007/BF00223491.

DOI:10.1007/BF00223491
PMID:2184346
Abstract

Localization of the regulatory subunit of cAMP-dependent protein kinase type II was studied in proliferating and quiescent fibroblasts 3T3 and in a cell line of neural origin pheochromocytoma PC12. In actively proliferating PC12 cells the regulatory subunit was found to be localized in the nucleus. Transition of these cells into a quiescent state was accompanied by a regulatory subunit translocation to the cytoplasm. In 3T3 cells the regulatory subunit was localized in the cytoplasm both in the quiescent and proliferating (though less actively than PC12 cells) states. Similar results were obtained both with monoclonal antibodies and with rabbit monospecific antiserum raised against the regulatory subunit type II from pig brain.

摘要

相似文献

1
Immunofluorescence localization of the regulatory subunit type II of cAMP-dependent protein kinase in PC12 and 3T3 cells in different proliferative states.
Mol Cell Biochem. 1990 Mar 5;93(1):47-52. doi: 10.1007/BF00223491.
2
Phosphorylation of tyrosine hydroxylase on at least three sites in rat pheochromocytoma PC12 cells treated with 56 mM K+: determination of the sites on tyrosine hydroxylase phosphorylated by cyclic AMP-dependent and calcium/calmodulin-dependent protein kinases.用56 mM K⁺处理的大鼠嗜铬细胞瘤PC12细胞中酪氨酸羟化酶至少三个位点的磷酸化：确定环磷酸腺苷依赖性蛋白激酶和钙/钙调蛋白依赖性蛋白激酶磷酸化的酪氨酸羟化酶位点
Mol Pharmacol. 1986 Nov;30(5):476-85.
3
[Study of the structural characteristics of the regulatory subunit of cAMP-dependent protein kinase type II using monoclonal antibodies].
Biokhimiia. 1987 Oct;52(10):1740-5.
4
A nerve growth factor-dependent protein kinase that phosphorylates microtubule-associated proteins in vitro: possible involvement of its activity in the outgrowth of neurites from PC12 cells.一种在体外使微管相关蛋白磷酸化的神经生长因子依赖性蛋白激酶：其活性可能参与PC12细胞神经突的生长。
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Neurosci Lett. 1980 Jul;18(3):333-7. doi: 10.1016/0304-3940(80)90307-9.
6
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[Use of monoclonal antibodies for purifying the regulatory subunit of cAMP-dependent protein kinase].[利用单克隆抗体纯化环磷酸腺苷依赖性蛋白激酶的调节亚基]
Biull Eksp Biol Med. 1987 May;103(5):567-9.
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Cell. 1985 Jul;41(3):1039-51. doi: 10.1016/s0092-8674(85)80084-2.
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Localization of pp60c-src in growth cone of PC12 cell.pp60c-src在PC12细胞生长锥中的定位。
Biochem Biophys Res Commun. 1988 Dec 30;157(3):1383-9. doi: 10.1016/s0006-291x(88)81028-3.

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本文引用的文献

1
Photoaffinity labeling of a microtubule-associated cyclic AMP-binding protein.一种微管相关环磷酸腺苷结合蛋白的光亲和标记
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2
[Nuclear translocation and effect of cAMP-dependent protein kinase on transcription].
Biokhimiia. 1980 Jun;45(6):979-91.
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Hypothesis. Cyclic AMP and its receptor protein in tumor growth regulation in vivo.假设。环磷酸腺苷及其受体蛋白在体内肿瘤生长调节中的作用。
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4
Protein kinase translocation following beta-adrenergic receptor activation in C6 glioma cells.C6胶质瘤细胞中β-肾上腺素能受体激活后的蛋白激酶易位
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Lac DNA, RNA polymerase and cyclic AMP receptor protein, cyclic AMP, lac repressor and inducer are the essential elements for controlled lac transcription.乳糖操纵子DNA、RNA聚合酶、环腺苷酸受体蛋白、环腺苷酸、乳糖阻遏物和诱导物是乳糖转录调控的基本要素。
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Ultrastructural immunocytochemical localization of cyclic AMP-dependent protein kinase regulatory subunits in rat parotid acinar cells.大鼠腮腺腺泡细胞中环磷酸腺苷依赖性蛋白激酶调节亚基的超微结构免疫细胞化学定位
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Synaptogenesis of cultured striatal neurons in serum-free medium: a morphological and biochemical study.无血清培养基中培养的纹状体神经元的突触发生：一项形态学和生物化学研究。
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Cyclic-AMP-dependent protein kinase type II is associated with the Golgi complex and with centrosomes.II型环磷酸腺苷依赖性蛋白激酶与高尔基体复合体和中心体相关。
Cell. 1985 Jul;41(3):1039-51. doi: 10.1016/s0092-8674(85)80084-2.
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Activities of cAMP-dependent protein kinase and enzymes of 2',5'-oligoadenylate metabolism in NIH 3T3 cells deepening into the resting state.
Exp Cell Res. 1985 Apr;157(2):335-42. doi: 10.1016/0014-4827(85)90118-1.