Valentincic Natasa Vidovic, de Groot-Mijnes Jolanda D F, Kraut Aleksandra, Korosec Peter, Hawlina Marko, Rothova Aniki
Eye Hospital, University Medical Centre, Ljubljana, Slovenia.
Mol Vis. 2011;17:2003-10. Epub 2011 Jul 20.
To study the intraocular and serum cytokine and chemokine profile in patients with intermediate uveitis (IU) at various stages of inflammatory activity.
Institutional, prospective association study. Paired aqueous humor (AqH) and serum samples were collected from 36 consecutive IU patients and 10 controls. The concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12p70, tumor necrosis factor (TNF)-α, CC--chemokine ligand 5/regulated upon activation normal T-cell expressed, and secreted (CCL5/RANTES), CC--chemokine ligand 3/macrophage inflammatory protein 1alpha (CCL3/MIP-1α), CCL4/MIP-1β, and CC--chemokine ligand 2/monocyte chemotactic protein--1 (CCL2/MCP-1) were measured in both AqH and serum by multiplex immunoassay. Main outcome measures were serum and intraocular levels of the analyzed cyto- and chemokines.
Patients with IU had higher serum levels of TNF-α than non-uveitic controls (p<0.0001), whereas their AqH TNF-α levels did not show a difference (p=0.323). IU patients had higher intraocular levels of IL-1β, IL-6, IL-8, IL-10, IL-12p70 and CCL2/MCP-1 than the controls (p=0.020, 0.001, <0.0001, 0.005, 0.003, and 0.003, respectively). Active stages of IU were characterized by higher levels of IL-6, IL-8, CCL5/RANTES and CCL2/MCP-1 (p=0.003, <0.0001, 0.033, and 0.033, respectively). Higher levels of IL-6 and IL-8 were found in IU patients with cystoid macular edema (CME) compared to non-CME IU patients (p=0.026 and 0.012, respectively). Significant positive correlations between various observed mediators were present in the AqH of IU patients only.
Significantly elevated concentrations of multiple intraocular cytokines were found in IU patients, especially IL-6 and IL-8 in those with CME and active disease. In serum elevated TNF-α levels were observed in IU patients. Our findings improve the understanding of the pathogenesis of IU and contribute to the identification of factors which may contribute to the activity of IU.
研究中间葡萄膜炎（IU）患者在炎症活动不同阶段的眼内和血清细胞因子及趋化因子谱。
机构性前瞻性关联研究。从36例连续的IU患者和10例对照者中采集配对的房水（AqH）和血清样本。通过多重免疫测定法测量房水和血清中白细胞介素（IL）-1β、IL-6、IL-8、IL-10、IL-12p70、肿瘤坏死因子（TNF）-α、CC趋化因子配体5/活化正常T细胞表达和分泌调节因子（CCL5/RANTES）、CC趋化因子配体3/巨噬细胞炎性蛋白1α（CCL3/MIP-1α）、CCL4/MIP-1β和CC趋化因子配体2/单核细胞趋化蛋白-1（CCL2/MCP-1）的浓度。主要观察指标为所分析的细胞因子和趋化因子的血清及眼内水平。
IU患者的血清TNF-α水平高于非葡萄膜炎对照者（p<0.0001），而其房水TNF-α水平无差异（p=0.323）。IU患者的眼内IL-1β、IL-6、IL-8、IL-10、IL-12p70和CCL2/MCP-1水平高于对照者（分别为p=0.020、0.001、<0.0001、0.005、0.003和0.003）。IU的活动期以IL-6、IL-8、CCL5/RANTES和CCL2/MCP-1水平较高为特征（分别为p=0.003、<0.0001、0.033和0.033）。与非黄斑囊样水肿（CME）的IU患者相比，CME的IU患者IL-6和IL-8水平更高（分别为p=0.026和0.012）。仅在IU患者的房水中，各种观察到的介质之间存在显著正相关。
IU患者中发现多种眼内细胞因子浓度显著升高，尤其是CME和活动性疾病患者中的IL-6和IL-8。在IU患者血清中观察到TNF-α水平升高。我们的研究结果有助于加深对IU发病机制的理解，并有助于确定可能导致IU活动的因素。
