Nuffield Department of Medicine, Target Discovery Institute, University of Oxford, Roosevelt Drive, Oxford, OX3 7FZ, United Kingdom.
Mass Spectrom Rev. 2021 Jul;40(4):408-565. doi: 10.1002/mas.21651. Epub 2021 Mar 16.
This review is the ninth update of the original article published in 1999 on the application of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2016. Also included are papers that describe methods appropriate to analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation and arrays. The second part of the review is devoted to applications to various structural types such as oligo- and poly-saccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals. Much of this material is presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions and applications to chemical synthesis. The reported work shows increasing use of combined new techniques such as ion mobility and the enormous impact that MALDI imaging is having. MALDI, although invented over 30 years ago is still an ideal technique for carbohydrate analysis and advancements in the technique and range of applications show no sign of deminishing. © 2020 Wiley Periodicals, Inc.
这是对 1999 年发表的原始文章的第九次更新，该文章介绍了基质辅助激光解吸/电离（MALDI）质谱在碳水化合物和糖缀合物分析中的应用，并涵盖了截至 2016 年底的文献。本文还包括描述适用于 MALDI 分析的方法的论文，例如样品制备技术，即使电离方法不是 MALDI。本综述的第一部分涵盖了一般方面，例如 MALDI 过程的理论、基质、衍生化、MALDI 成像、碎片化和阵列。综述的第二部分专门讨论了各种结构类型的应用，如寡糖和多糖、糖蛋白、糖脂、糖苷和生物制药。这些材料中的大部分以表格形式呈现。综述的第三部分涵盖了该技术在医学和工业中的应用、酶反应研究以及在化学合成中的应用。报道的工作表明，越来越多地使用离子淌度等新技术，以及 MALDI 成像所产生的巨大影响。尽管 MALDI 技术发明已有 30 多年，但它仍然是碳水化合物分析的理想技术，该技术的进步和应用范围都没有减弱的迹象。© 2020 Wiley Periodicals, Inc.
