Optometry and Vision Research, Weinheim, Germany.
Cont Lens Anterior Eye. 2012 Feb;35(1):35-8. doi: 10.1016/j.clae.2011.07.005. Epub 2011 Aug 17.
To (i) analyze the repeatability of fluorescein instillation from a modified fluorescein strip (MFS) compared to a standard fluorescein strip (FS), and to (ii) observe its usefulness in the measurement of the fluorescein break-up time (FBUT) in comparison to the Tearscope (NIBUT).
In-vitro: Intra- and inter-observer repeatability in fluorescein instillation from the MFS and FS was evaluated by fluorescence analysis (n=10, each). In-vivo: BUT of the right eye of 20 randomly selected subjects (mean age 43.3±11.5, range=21-60 years, 8 males, 12 females) was measured by use of the Tearscope and MFS. Subjects were grouped by the Ocular Surface Disease Index (OSDI) score into 8 OSDI+ and 12 OSDI- by a cut-off value of 15.
In-vitro: Intra-observer 95% limit of agreement (LoA) of the MFS was similar to the FS LoA in observer 1 (O1), but better than the FS LoA in observer (O2) (MFS: O1: LoA=±1.98 mW; p=0.179; O2: ±2.71; 0.442; FS: O1: ±1.71; 0.246; O2: ±4.11; 0.512). Inter-observer LoA in fluorescence was better in MFS (±1.42; 0.111) than in FS (±3.71; 0.003). In-vivo: MFS-BUT was significantly shorter than the NIBUT (p=0.002), but significantly correlated (r=0.864, p<0.001). NIBUT and MFS-BUT were significant discriminators (p<0.001) of OSDI±(0.948/8s and 0.938/5s [AUC/cut-off value]; NIBUT and MFS-BUT, respectively).
The MFS was better in the repeatability of fluorescein instillation than the FS. NIBUT and MFS-BUT were good discriminators of dry eye symptoms, but differ in their cut-off values.
（i）分析改良荧光素条（MFS）与标准荧光素条（FS）相比在荧光素滴眼方面的可重复性，并（ii）观察其在测量荧光素泪膜破裂时间（FBUT）方面与泪液干涉仪（NIBUT）相比的有用性。
在体外：通过荧光分析评估 10 名（每位）观察者内和观察者间 MFS 和 FS 荧光素滴眼的可重复性。在体内：使用泪液干涉仪和 MFS 测量 20 名随机选择的受试者（平均年龄 43.3±11.5 岁，范围 21-60 岁，男性 8 名，女性 12 名）右眼的 BUT。根据眼表疾病指数（OSDI）评分，受试者分为 OSDI+组（8 名）和 OSDI-组（12 名），分界值为 15。
在体外：观察者 1（O1）的 MFS 观察者内 95%限差（LoA）与 FS LoA 相似，但优于观察者 2（O2）的 FS LoA（MFS：O1：LoA=±1.98 mW；p=0.179；O2：±2.71；0.442；FS：O1：±1.71；0.246；O2：±4.11；0.512）。荧光内观察者间 LoA 在 MFS 中更好（±1.42；0.111），而在 FS 中更差（±3.71；0.003）。在体内：MFS-BUT 明显短于 NIBUT（p=0.002），但具有显著相关性（r=0.864，p<0.001）。NIBUT 和 MFS-BUT 是 OSDI±（0.948/8s 和 0.938/5s[AUC/截断值]）的显著鉴别器（p<0.001）。
MFS 在荧光素滴眼的重复性方面优于 FS。NIBUT 和 MFS-BUT 是干眼症症状的良好鉴别器，但截断值不同。
