用鼻探头刮匙获取的鼻咽标本中呼吸道合胞病毒的快速检测。
Rapid detection of respiratory syncytial virus in nasopharyngeal specimens obtained with the rhinoprobe scraper.
作者信息
Jalowayski A A, Walpita P, Puryear B A, Connor J D
机构信息
Department of Pediatrics, University of California, San Diego.
出版信息
J Clin Microbiol. 1990 Apr;28(4):738-41. doi: 10.1128/jcm.28.4.738-741.1990.
Abstract
We compared the Rhinoprobe scraping technique for collection of superficial nasal mucosa epithelial cells and rapid detection of respiratory syncytial virus by immunofluorescence with paired, swab-collected specimens for virus culture from 1,257 infants and children with acute respiratory infections. Compared with viral culture as the reference test, the sensitivity, specificity, and accuracy of the immunofluorescence test were 83.6, 93.6, and 91.3%, respectively. We found the Rhinoprobe method safe, easy to use, and helpful in obtaining large quantities of epithelial cells for detection of respiratory syncytial virus and other respiratory viruses.
摘要
我们对1257名患有急性呼吸道感染的婴幼儿，比较了用于收集鼻黏膜表层上皮细胞的Rhinoprobe刮取技术以及通过免疫荧光法快速检测呼吸道合胞病毒的方法与配对的、用拭子采集的用于病毒培养的标本。以病毒培养作为参考检测方法，免疫荧光检测的敏感性、特异性和准确性分别为83.6%、93.6%和91.3%。我们发现Rhinoprobe方法安全、易用，有助于获取大量上皮细胞以检测呼吸道合胞病毒和其他呼吸道病毒。
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