Treuhaft M W, Soukup J M, Sullivan B J
J Clin Microbiol. 1985 Aug;22(2):270-3. doi: 10.1128/jcm.22.2.270-273.1985.
In our private clinic-hospital setting, respiratory syncytial virus (RSV) was isolated from infants more frequently and sooner from nasal washes (84%; 4.2 days) than from throat swabs (45%; 5.5 days) or nasopharyngeal swabs (39%; 5.7 days). Immunofluorescence of nasal wash cells identified 72% of the infants with virus isolations from nasal washes in less than one day. We therefore recommend the combination of isolation and immunofluorescence on nasal wash specimens for optimal detection of RSV-infected infants. Immunofluorescence of respiratory tract cells was also useful for monitoring the presence of RSV antigen in intubation secretions during ribavirin antiviral therapy. RSV infectivity was maintained in phosphate-buffered saline at room temperature for 6 h. Transport and inoculation of specimens in less than 6 h yielded RSV isolates from 50% of sampled infants during the two RSV seasons examined. For optimal RSV isolation, we recommend inoculation of HEp-2 tubes less than or equal to 4 days old. Replacing medium after 3 days as compared with 7 days did not increase recovery of RSV and provided little practical reduction in time to detection of cytopathology.
在我们的私立诊所-医院环境中，从婴儿鼻腔冲洗液中分离出呼吸道合胞病毒（RSV）的频率更高、时间更早（84%；4.2天），高于咽喉拭子（45%；5.5天）或鼻咽拭子（39%；5.7天）。鼻腔冲洗液细胞的免疫荧光检测发现，72%从鼻腔冲洗液中分离出病毒的婴儿在不到一天的时间内被检测出来。因此，我们建议对鼻腔冲洗液标本进行分离和免疫荧光检测相结合，以实现对RSV感染婴儿的最佳检测。呼吸道细胞的免疫荧光检测对于监测利巴韦林抗病毒治疗期间插管分泌物中RSV抗原的存在也很有用。RSV在室温下于磷酸盐缓冲盐水中可保持感染力6小时。在两个RSV流行季节中，在不到6小时内对标本进行转运和接种，50%的采样婴儿获得了RSV分离株。为实现最佳的RSV分离，我们建议接种年龄小于或等于4天的HEp-2管。与7天后更换培养基相比，3天后更换培养基并没有提高RSV的回收率，且在检测细胞病变的时间上几乎没有实际缩短。
