Rossney A S, English L F, Keane C T
Microbiology Department, St James's Hospital, Dublin, Ireland.
J Clin Pathol. 1990 Mar;43(3):246-52. doi: 10.1136/jcp.43.3.246.
Five commercial Staphylococcus aureus identification kits--Staphaurex (Wellcome), Staphylase (Oxoid), Staphyslide (bioMèrieux), Biostaph (Medlabs) and Bacto Latex (Difco)--were evaluated for the routine identification of S aureus from primary plates in the routine microbiology laboratory. Comparison was made with two methods of tube coagulase testing and five slide methods for detecting clumping factor (slide coagulase testing). Performances were assessed for two groups of organisms, staphylococcal species alone and a combined staphylococcal and non-staphylococcal species group. The effects of growth on selective media and storage of isolates at room temperature and 4 degrees C were investigated. Selective media cannot be recommended, nor can storage of isolates before testing. Ranked according to efficiency value with the combined staphylococcal and non-staphylococcal species group, the kits and coagulase methods performed as follows (the figures in parentheses are the efficiency values for the staphylococcal group alone): tube coagulase reference method 100% (100%), tube coagulase SJH method 99% (99%), Staphaurex 94% (97%), Staphylase 93% (96%), slide coagulase method No 4 93% (94%), slide coagulase method No 5 93% (93%), Bacto Latex 92% (95%), Staphyslide 92% (95%), and Biostaph 87% (91%). It is concluded that a commercial S aureus identification kit should not replace tube coagulase testing for the routine identification of the organism from primary plates and that, even the kits with the best performances, have little advantage over a good slide coagulase test method.
对五种市售金黄色葡萄球菌鉴定试剂盒——Staphaurex（威康公司）、Staphylase（奥克托公司）、Staphyslide（生物梅里埃公司）、Biostaph（Medlabs公司）和Bacto Latex（迪夫科公司）——进行了评估，以用于常规微生物实验室从原始平板中对金黄色葡萄球菌进行常规鉴定。将其与两种试管凝固酶检测方法以及五种检测凝聚因子的玻片法（玻片凝固酶检测）进行了比较。对两组微生物进行了性能评估，一组仅为葡萄球菌属菌种，另一组为葡萄球菌属菌种与非葡萄球菌属菌种的组合。研究了在选择性培养基上生长以及在室温及4℃下保存分离株的影响。不推荐使用选择性培养基，也不建议在检测前保存分离株。根据葡萄球菌属菌种与非葡萄球菌属菌种组合组的效率值进行排序，各试剂盒和凝固酶方法的表现如下（括号内数字为仅葡萄球菌属菌种组的效率值）：试管凝固酶参考方法100%（100%），试管凝固酶SJH方法99%（99%），Staphaurex 94%（97%），Staphylase 93%（96%），玻片凝固酶方法4号93%（94%），玻片凝固酶方法5号93%（93%），Bacto Latex 92%（95%），Staphyslide 92%（95%），Biostaph 87%（91%）。得出的结论是，对于从原始平板中对该菌进行常规鉴定，市售金黄色葡萄球菌鉴定试剂盒不应取代试管凝固酶检测，而且，即使是性能最佳的试剂盒，与良好的玻片凝固酶检测方法相比也没有太大优势。
