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基于 RD 抗原的纳米疫苗通过诱导针对实验性鼠结核的记忆应答来提供长期保护。

RD antigen based nanovaccine imparts long term protection by inducing memory response against experimental murine tuberculosis.

机构信息

Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.

出版信息

PLoS One. 2011;6(8):e22889. doi: 10.1371/journal.pone.0022889. Epub 2011 Aug 11.

DOI:10.1371/journal.pone.0022889
PMID:21853054
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3154911/
Abstract

BACKGROUND

The absence of certain genomic loci that are present in most of the virulent strains of Mycobacterium tuberculosis as well as lack of lasting memory responses are some of the major causes attributed to the non effectiveness of Bacille Calmette-Gue'rin (BCG) vaccine. Immunization schedules addressing these issues can offer better strategy for protection against tuberculosis.

METHODS

The immunological responses evoked upon administration of archaeosome based antigen delivery system comprising T cell antigen, Rv3619c (an ESAT-6 family protein), has been assessed against experimental murine tuberculosis in BALB/c mice.

RESULTS

Archaeosome based subunit vaccine has been found to elicit type-1 cytokines in the immunized mice. Besides effective T cell memory response, the Rv3619c based vaccine was able to reduce mycobacterial burden in the animals challenged with Mycobacterium tuberculosis infection.

CONCLUSION

The data of the present study suggest that archaeosome encapsulated RD gene products offer substantial protection against M. tuberculosis infection.

摘要

背景

结核分枝杆菌毒力株中存在某些基因组位点的缺失，以及缺乏持久的记忆应答，这是导致卡介苗（BCG）疫苗效果不佳的主要原因之一。针对这些问题的免疫接种方案可以提供更好的保护结核病的策略。

方法

在 BALB/c 小鼠中评估了含有 T 细胞抗原、Rv3619c（一种 ESAT-6 家族蛋白）的基于类病毒体的抗原递呈系统给药后引起的免疫反应，以对抗实验性小鼠结核病。

结果

基于类病毒体的亚单位疫苗已被发现可在免疫小鼠中诱导产生 1 型细胞因子。除了有效的 T 细胞记忆应答外，基于 Rv3619c 的疫苗还能够减少感染结核分枝杆菌的动物中的分枝杆菌负荷。

结论

本研究的数据表明，类病毒体包封的 RD 基因产物可提供针对 M. tuberculosis 感染的实质性保护。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/be0f0c458ead/pone.0022889.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/d1ab5ddadea5/pone.0022889.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/03a32913f954/pone.0022889.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/f3e11487e7e1/pone.0022889.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/fac43567f892/pone.0022889.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/566c157ee367/pone.0022889.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/27a8bfba248a/pone.0022889.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/be0f0c458ead/pone.0022889.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/d1ab5ddadea5/pone.0022889.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/03a32913f954/pone.0022889.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/f3e11487e7e1/pone.0022889.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/fac43567f892/pone.0022889.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/566c157ee367/pone.0022889.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/27a8bfba248a/pone.0022889.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259e/3154911/be0f0c458ead/pone.0022889.g007.jpg

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